Summaries Published in the November 2018 Issue
Jan Koschorreck, German Environment Agency; Elisabeth Nyberg, Swedish Museum of Natural History; and Sara Danielsson, Swedish Museum of Natural History
Environmental specimen banks (ESBs) systematically store high-quality samples from the environment and human populations in support of chemicals management and innovative research for a better environmental quality. Still, there is a large potential to make better use of ESB samples in research and chemicals regulation.
There are around 30 ESBs in the world, of which about 20 are located in Europe, and for some ESBs, sampling has been ongoing for up to 50 years at regular intervals. The specimens are used to monitor the quality of the environment and the efficacy of regulatory efforts to control known hazardous substances like metals, polyaromatic hydrocarbons (PAHs), polybrominated diphenylethers (PBDEs), dioxins and other organochlorines or -halogens. However, the main objective of ESBs is retrospective analysis of chemicals of emerging concern (CECs): ESB samples allow for spatio-temporal analysis of substances that are unknown, not known to be hazardous or not analytically detectable at the time of sampling. Recent examples are new per- and polyfluorinated substances (PFAS), new flame retardants, chlorinated paraffins (CPs), nanomaterials and pharmaceuticals.
The systematic use of high-quality ESB data and samples has the potential to significantly increase our understanding of the fate of regulated and non-regulated contaminants in the environment. ESB data is already being used to some extent to identify persistent organic pollutants (POPs) under the Stockholm Convention and potential persistent and bioaccumulative compounds in the European REACH legislation. However, consistent and interdisciplinary approaches that make better use of high-quality ESB samples can overcome the reluctant use of monitoring data in chemicals risk assessment.
The application of cutting-edge analytical methods to ESB samples is a win-win situation for the value of ESB samples and our understanding of the quality of the environment. For example, non-target screening is a promising tool that will add significant data on the temporal occurrence of chemicals in ecosystems. Some archived samples are now also used for barcoding and meta-barcoding approaches of environmental eDNA revealing temporal changes in biodiversity and offering interdisciplinary links to this research area. Furthermore, banked environmental and human samples can be incentives for interdisciplinary and integrated exposure assessments.
The aim of this ESB session was to discuss interdisciplinary applications for ESB samples and to initiate collaborations with environmental scientists and regulators in the field of long-term environmental quality monitoring. The invited speakers represent both newer and well established ESBs as well as ESBs focusing on different types of samples. Short summaries of the five presentations are given below.
- Temporal trends of POPs in the Swedish aquatic ecosystem and in human milk were presented by Elisabeth Nyberg, Swedish Museum of Natural History, and she concluded that the non-linear trends differ between monitoring matrices for several contaminants. In some cases, the peak differs, and in others, the concentration is leveling out for one matrix but continues to decrease for another. In addition, the concentrations of polychlorinated biphenyls (PCBs), dichlorodiphenylchloroethanes (DDTs) and hexachlorocyclohexanes (HCHs) are, despite continuous decreases since the 1970s, still higher in the Baltic Sea compared with, for example, the North Sea.
- Anne Dreyer, Eurofins, presented results for conventional and novel or alternative flame retardants in environmental samples from the German ESB in time series going back to the 1980s. She concluded that PBDEs, emerging brominated flame retardants (eBFRs) and dechloranes were observed in the analyzed samples but that concentrations for almost all target compounds were decreasing over time. Dreyer thus posed the questions if the eBFRs and dechloranes really are emerging and, perhaps, which other substitutes for regulated flame retardants should we be focused on?
- Pernilla Bohlin-Nizzetto, Norwegian Institute for Air Research (NILU), highlighted the importance of controlling the indoor laboratory environment to avoid sample contamination. Selected target screening results from passive air samplers and dust show that several of the non-regulated chemicals of emerging concern are found at high levels in the indoor matrices. These include CPs, new flame retardants and volatile methylsiloxanes. In contrast, regulated contaminants (e.g., PCBs and PBDEs) are found at low levels.
- Results, both temporal and spatial, from the screening of perfluoroalkyl acids (PFAAs) and precursors in various samples from the German ESB were presented by Matthias Kotthoff, Fraunhofer IME. Perfluoropropionic acid (PFPrA) was for the first time detected in several biotic matrices similar to a PFAS produced in China (F-53B), which was detected for the first time in European biota. The results show that it is of high importance to increase the spectrum of analyzed PFAS and that the focus should be on short chain and ether PFAS and precursor molecules.
- Jonas Astrin, Zoological Research Museum A. Koenig, discussed the importance of ESBs following defined routines since they constitute a very valuable source of DNA for biodiversity research, as they keep open a window that allows the parallel, correlative analysis of the chemical and of the species community composition of a given environment over time. Thus, by adding DNA banking to their service spectrum, ESBs can considerably increase their visibility and public demand. Furthermore, they can foster knowledge aggregation at the biodiversity level around their ESB samples, making these more valuable.
The session ended with a 15 minute discussion on future interdisciplinary applications for ESBs.
The results presented in this session and the following discussion made it clear that the use of ESB data and samples has in many cases increased the knowledge on both regulated and non-regulated contaminants in the environment. However, there is a large potential to make better use of ESB samples in research and chemicals regulation by increasing the understanding of the potential of ESBs both in the research community and by the general public.
Non-target screening (NTS) was regarded to have a big potential for ESBs since it is possible to store the data from matrices that are difficult to archive, e.g., water and air. The resulting virtual ESB data archives can be seen as complementary to traditional ESBs with biota and other environmental specimens. Furthermore, the use of mass spectrometric suspect screening approaches allows to characterize the contamination of samples for a much larger number of chemicals than previously possible. It is expected that NTS analysis of ESB samples will greatly improve our understanding of the historical evolution of chemical contamination. A representative of the University of the Basque Country mentioned another example where novel techniques improve the analysis of banked samples: Images from marine biota histological samples are stored as digital samples with open access to scientists via the internet.
Regulators at the ESB session mentioned that environmental monitoring data are being used more, in both substance risk assessment and effectiveness evaluation of chemical regulation. The Stockholm Convention was mentioned as the example for the use of trend data from different regions. For the EU, REACH was mentioned as a relevant regulation where ESBs could contribute with data (identification of substances of very high concern). An example is the identification of possibly biomagnifying current-use chemicals by analysis of biota from several trophic levels of an ecosystem and determination of trophic magnification factors (TMFs). ESBs with an appropriate sampling strategy could deliver samples for such a study on demand.
Charmaine Ajao, Thomas-Benjamin Seiler and Annegaaike Leopold; Session Co-chairs
Gunilla Öberg, one of the speakers, presented the take-home message from our well-attended special session at the SETAC Europe 28th Annual Meeting in Rome,“We as scientists should show awareness, humility and courage in developing, performing and communicating our environmental research.” The hall, with a capacity of 300 chairs, was full throughout both parts of the session, just before and just after lunch, with people even sitting on the floor or standing. The organizers received many positive reactions during and after the session. One attendee said words to the following effect, “I have been coming to SETAC meetings for many years now and this is the first time that I have found what I am looking for – very inspiring.” The ECHA presentation given by Charmaine Ajao was also commended by the editor of the Integrated Environmental Assessment and Management (IEAM) SETAC journal.
Creating awareness through improvement of science and risk communication to and among stakeholders was the topic that was given highest priority by a group of environmental science stakeholders who attended the Horizon Scanning Project Stakeholder Event during the SETAC Europe 27th Annual Meeting in Brussels in May 2017. This is what triggered the development of this session.
The session followed a unique structure. After a more general and practical talk on science communication, it featured speakers presenting as a duo, and in one case as a trio, with very succinct five- to ten-minute “point and counter point” talks around three selected themes of emerging concern to scientists, the public and regulators alike: Endocrine disruptors, nanomaterials and (micro)plastics.
Sofie Vanthournout, from Sense About Science EU, was welcomed as a breath of fresh air by the SETAC audience, shaking us up with five top tips for how we, as environmental researchers, can work in alliance with citizens to fight misinformation and improve public debates. The tips included:
- To communicate WITH the public rather than TO the public
- To respect the audience and public and not to underestimate them – the public is not stupid but is most likely misinformed
- To listen to the public’s concerns and be willing to agree to disagree
- To be humble and honest about your limits of expertise, especially since public debates are never focused on a single topic
- To talk about trade-offs and explain the bigger picture.
During the discussion with the audience, the key question was how to ensure that research is independent. It was argued by Vanthournout that placing several people around the table to represent different perspectives is the way forward. Others argued that funders of research determine which research is done. A logical response to that was that scientists should be lobbying with the funding agencies. It was also pointed out that success stories should be told and scientists should not only focus on the uncertainties.
This was followed by a duo presentation on how to communicate the risks posed by endocrine disrupting chemicals by Juliette Legler, Utrecht University, who represented the human safety aspects, and Markus Hecker, University of Saskatchewan, who represented the environmental safety aspects. Mixed messages in the media, in scientific publications, as well as regional differences in regulation and decision making, for example the hazard approach to endocrine disruptors in Europe and the risk approach to the same issue in North America, can lead to the public mistrusting the science. Legler and Hecker highlighted that scientists, when communicating with the public, often take on the role of informing the public after their research results have been generated, which can lead to fear. Instead they are advised to interact with the public and engage the public from the outset of the research. They proposed to have more informed co-creation of research with the public, very much in line with Vanthournout’s message. A practical Canadian example given during the talk was that to reach out to the public, scientists communicate and discuss their findings in open meetings in bars. In their talk, they also referred to regulators as one main stakeholder in science communication. In their eyes, regulators are faced with an information overload, and hence, they rely more and more on briefs and reviews that may be provided by the media. Scientists should realize that they are responsible for providing robust information to decision makers who need to take decisions based on scientific evidence. Legler and Hecker suggested to “cut out the middle man” (i.e., the media) where possible and provide our own briefs and press releases. Training scientists in science communication is therefore very important.
Charmaine Ajao, ECHA, gave a regulator’s perspective to involving stakeholders and the public in the regulation of a substance. This presentation provided a practitioner’s response to the question raised by the duo before this presentation of how decisions are taken by decision makers. This presentation explained the lines of evidence considered by ECHA in the regulation of a substance. A case example from Member State Committee (MSC) agreement-seeking in substance evaluation was presented, where evidence from the Registrant following a proposal for amendments led to a change in the testing strategy requested by the evaluating Member State and subsequent agreement by MSC. The presentation explained the regulatory boundaries of decision makers and the iterations made with different stakeholders to arrive at a transparent, independent decision based on scientific evidence.
After lunch Fabienne Schwab, from the Adolphe Merkle Institute, opened the floor. She spoke from the perspective of scientists working in the lab, who publish their results, only to have the media put a negative spin on their results. She asked the question how scientists can correct the negative message. Schwab shared her personal experience of when media took hold of a scientific publication in which she reported that “nanotubes ‘rob’ green algae of space and light.’’ This led to several reliable articles on about 10 websites and requests for the original publication. However, in a few articles the research results were misrepresented by the media, the most extreme example being an online article which spoke about “Nanoparticles Identified as Potential Environmental Killers.” Schwab encouraged those that had similar experiences to take social responsibility, take corrective actions, follow the responses and fight for the message to be corrected when misquoted or misinterpreted.
Following on from and complementing Schwab’s presentation, Gunilla Öberg, from the University of British Columbia, spoke of the importance of knowing your audience and not conflating the interested public with the general public. While the general public is best reached through daily media, the interested public is best reached through specialized outlets, such as The Conversation, Sense about Science and AGUs Sharing Science. This is because in the eyes of daily media, “newsworthiness” means high emotional arousal, where the four A’s are: anxiety, amusement, anger and awe. When it comes to chemicals, anxiety is undeniably the most likely angle, and it is crucial that you are aware that this is probably how your research will be pitched if you send your press release to daily media. This brought us back to the message of the morning’s speakers: We need to communicate with and not to the public.
The final part of the session was a trio presentation focused on the communication about micro- and nanoplastics. Michiel Kotterman, Wageningen Marine Research (WMR) – Wageningen University & Research, was invited because the co-chairs believed that he maintains “a good portion of skepticism regarding the environmental relevance of micro- and nanoplastics, at least nowadays” and because he goes against mainstream opinion. Clearly, any action that stops people polluting the environment with waste, e.g., plastics, should be encouraged. However, he made a strong case for science investigating real and relevant dangers, as to date the general public has been receiving a much more dramatic impression of the dangers of plastics than has been scientifically demonstrated. In this context, Kotterman warned against the ease with which funding for plastic research can be attained, even for sloppy research. He illustrated that plastic research is a good example of what is wrong with funding science: He argued that claiming catastrophic effects of plastics, using public opinion, achieves funding, while presenting a balanced project about possible effects does not. Martin Wagner, Norwegian University of Science and Technology, continued by looking at whether scientists are communicating the risks of microplastics in the right way. He explained to us that the public has already decided what to conclude. It is up to us as scientists not to fight the public opinion but to work with the public to research the questions they have and do what they are asking us to do. Science may not be the only “tool’’ we have to address the issue. It was important in informing the public that plastic was ubiquitous and persistent. However, other factors – irreversibility, visibility, aesthetics, moral societal context – all need to be considered as well, and these are not solely within the purview of scientists. Finally, Angel Borja, AZTI, explained the efforts that scientists from the EU project ResponSEAble are putting in to try to change the attitude of the public through ocean literacy. This trio presentation ended by showing a two-minutes video on micro- and nanoplastic pollution that is being used to raise awareness to the public. This video in itself led to sharp criticism from members of the audience, which made the session all the spicier!
From the panel discussions that were held in both parts of the session, a general agreement emerged among the participants in the room that more efforts need to be made to communicate with the public rather than to the public and to involve the public in the formulation of the research questions. Secondly, awareness was raised for the academia scientists to work more with the industry scientists. It was recognized again that absence of evidence is not evidence of absence. However, in a current culture of polarization, it was accepted that it is quite difficult to find middle ground both with politicians (e.g., those who believe that there is no climate change) and with the public. It is our responsibility as scientists to show awareness, humility and courage to ensure that reliable science is performed in good communication with the public, the media and the decision makers about the concerns and interests of the public, the findings of the researchers, and the remaining risks and uncertainties.
Ana I. Catarino, Heriot-Watt University; Maya Al Sid Cheikh, University of Plymouth; and Farhan R. Khan, Roskilde University
Fragmentation of plastic and tire rubber debris and their release in aquatic environments are issues of concern due to their impact with the biota. Current environmental risk assessment strategies developed for solutes are likely inadequate for particles as the fate of particles is governed by the kinetic principles of aggregation and transport. At the SETAC Europe 28th Annual Meeting in Rome, we discussed the analytical challenges of detecting environmental concentrations of micro- (MPs) and nanoplastics (NPs) and reflected upon the contribution of rubber particles to the “plastic soup” in aquatic environments. The session was represented by a total of six oral presentations, six poster-corner presentations and 28 posters from researchers across the globe.
Particle Production and Analytical Detection Challenges
Despite the numerous concerns regarding the potential negative effects on aquatic biota of both micro- and nanoplastics, the establishment of baseline levels in the environment is still a difficult task. Presenters such as Gabriella Schirinzi, IDAEA-CSIC, discussed solutions to challenge the problematics related to the environmental identification of these particles such as variability in terms of molecular weight, density, low solubility, laboratory contamination, among others, and to establish standard observation protocols. Quantitative and qualitative analysis of environmental samples can be achieved using a combination of analytical techniques (i.e., Thermogravimetric Analysis, Differential Scanning Calorimetry, Fourier-Transformed Infrared Spectroscopy, Atmospheric Pressure Chemical Ionization), but a recurrent concern in most presentations was the need for standardization of observation methodologies and testing materials (Julio Sanchez Nieva, University of Cadiz). The affordable and accessible production of plastic particles to be tested in micro- and nanoplastics experimental work, including analytical studies, is a valuable feature under development. Diversity in terms of particles polymers and shapes is desirable to mimic the diversity of plastic contamination in the environment. Gireeshkumar Balakrishnan Nair, Institute of Materials and Molecules of Le Mans, presented strategies to produce < 1 µm polyethylene (PE) particles, whereas Susanne Kuehn, Wageningen Marine Research, discussed the preparation of realistic weathered < 3 µm litter particles, with both studies highlighting the use of analytical tools to validate their methodology. The application of nuclear techniques has been proposed as a solution to measure low concentrations of microplastics in distinct environmental compartments, and for evaluating biokinetics as well as toxicodynamic interactions with co-contaminants (Marc Metian, International Atomic Energy Agency/Radioecology Lab), emphasizing the need to develop further this study area.
Environmental Detection and Biota and Human Exposure
Assessment of microplastics impacts in aquatic organisms can be challenging if detection methods are not well developed to accurately quantify ingestion, retention and egestion rates. Beatriz Fernández, Instituto Español de Oceanografia, presented and discussed solutions to assess the uptake kinetics for microplastics (3-20 µm) in organisms using techniques such as quantification via a Multisizer Coulter Counter. A major apprehension of the public is on the exposure humans can have to plastic micro- and nanoparticles. This has led to the development of methodologies for detection of inhalable-size microplastics, especially in urban areas (Joseph Levermore, Kings College London) and the investigation of particles that can be found in food, for instance via table salt (Ji-Su Kim, Incheon National University). We finalized our discussion with a reflection on the definition and characterization of microplastics and nanoplastics, having in mind the learnings that can be taken from nanoparticles expertise.
Tire Rubber Debris
Nine presentations (two platform, seven poster) highlighted the emergent research area of car tire rubber emissions. Although recognized as a major contributor to the environmental microplastic burden (Rachel Leads, College of Charleston, and Dorte Herzke, Norwegian Institute for Air Research), tire rubber has received comparatively little attention. Both the analytical challenges associated with identifying tire particles in the environment and the potential ecotoxicological impacts on aquatic organisms were covered. Paul Eisentraut, Bundesanstalt für Materialfoschung und -prüfung, and Phillipp Klöckner, Helmsholtz Centre for Environmental Research, described novel methods for identifying tire rubber from environmental samples based on the chemical marker compounds. The former method indicated the use of several potential markers, including elastomers, whereas the latter talk looked specifically at the presence and ratio of S and Zn. Research regarding toxicological effects is still in its infancy, but studies with aquatic test species, such as grass shrimp (Palaemonetes puglo) (John Weinstein, The Citadel) and Hyalella azteca (Louise Lynn Halle and Farhan Khan, Roskilde University) did indicate that the exposure to car tire particles could result in a variety of deleterious effects.
This was a very well-attended session, which reflects the major concerns around plastic pollution and biota and human exposure. Attendees raised highly pertinent questions, facilitating a productive and stimulating discussion. Only by appreciating the challenges that still lie ahead in detecting and quantifying realistic emissions of plastic and tire rubber particles in aquatic ecosystems, can we find creative and practical solutions. These solutions will be critical to accurately assess environmental impacts and risks, and to answer the current concerns of both the general public and policymakers.
Thomas-Benjamin Seiler and Leonie Nüßer, RWTH Aachen University, Institute for Environmental Research
SETAC research, i.e., all the research conducted by the SETAC members, matters to the people, stakeholders and policy makers. However, since it is so close to the expectations, opinions, fears and desires of our target audiences, environmental research is difficult to communicate. These topics directly touch the sense of well-being of our audience and, hence, are often taken on more emotional than is actually necessary – or better still even helpful. They have a tendency to go “viral” on social media platforms. Conversely, topics which may be perceived as prosaic can often be under-reported and evade the popular consciousness; e.g., topics that strongly matter to mankind but are perceived as inconvenient or even superfluous, such as sustainability. As a consequence, findings from environmental research can be readily misunderstood, or simply ignored, when not communicated in a proper way.
For this session we invited submissions that could tell a success story and lessons learned about science and risk communication, which depicted a communication concept in a research proposal, presented cutting-edge communication research, summarized experience in communicating science over the last couple of years or the entire career, introduced new ideas on how to successfully communicate our research, or described a best practice in communication about environmental sciences and risks. We asked all presenters to answer this one question: Do you think it’s worth it?
Unfortunately, science and risk communication is often neither an element of education when becoming an environmental scientist, nor part of many scientist’s job description; let alone that there is seldom time left for this on a daily basis. Consequently, yearly sessions on science and risk communication suffer from a rather low number of submissions. This was again the case for the annual meeting in Rome, which is why our session was organized as a pure poster corner.
However, this was actually not to the disadvantage of the session. Poster corners are a really nice, interactive and entertaining format. That is, if you as chair(s) like stages. Then you can turn a poster corner into a real event, with exciting short talks, vivid discussions and much attention and visibility by a quite large audience.
In Rome, we had a poster corner with nine instead of the usual five to six posters. These were all the abstracts that had been submitted to our session, and by utilizing the poster corner format, we were able to accommodate them all. Each presenter could choose between using all the time available to them for their presentation and postpone the discussion to after the poster social, or making it short and saving time for questions and a brief discussion directly after the presentations. Interestingly, all presenters chose to use the time they had and discuss after the session. This could indicate that (a) SETAC scientists are not prepared to give short, few minutes talks and (b) few minutes for discussion in a tight session schedule are considered not attractive for scientific exchange.
The session featured reports on research studies, such as the poster by Ursula Klaschka from the Ulm University of Applied Science, who stated that the majority of consumers do not understand risk communication instruments as intended by legislators. They presented a study that showed that well-informed and educated “best case” consumers do not frequently use information distributed by authorities but rather other sources of information. The study found that consumers need help to understand risk communication instruments about how to determine which products might contain harmful substances. Gretchen Bielmeyer-Fraser from Jacksonville University reported on the success of the research project STEAM (Science, Technology, Engineering, Arts, Math), focused on graduate and under-graduate interdisciplinary research in the fields of environmental science and communication, where the resulting data were publicized using modern communication tools such as social media. The presenters explained how the collaboration ultimately secured funding and successfully incorporated service learning and research opportunities for students. Matthieu Mondou from McGill University explored the role of SETAC in policy learning. He presented survey data collected from participants in previous SETAC forums. The study summarized what respondents believe regarding instrumental and core policy concerning alternative testing methods. It then assessed their self-reported policy learning experiences at SETAC and evaluated the role of SETAC as a professional forum that helps to learn and adapt to emerging challenges in regulatory science.
Other presenters focused on platforms and frameworks that enable communication or help improving outreach activities. Abdelqader Sumrein of the European Chemicals Agency (ECHA) introduced the European Union Observatory for Nano Materials (EUON), a new platform by ECHA for communicating information on the safety of nanomaterials. The website targets, among others, the general public, regulators, industry and scientists, and aims to increase the transparency of nanomaterials research. Tomas Rydberg from the Swedish Environmental Research Institute (IVL) reported on the development of a framework standard on monetary valuation of environmental impacts from human activity. The goal is to improve transparency of money valuation and its use in management.
Two posters presented knowledge and experience about specific tools for communication. Amy Oen from the Norwegian Geotechnical Institute suggested Living Labs as a means to involve a range of committed stakeholders in order to enhance the value of climate data. This work is part of the EVOKED project. As Oen pointed out, this approach allows engaging end-users and including their contribution for a co-creation of climate services. The framework that the project applies encourages end-users to share their perceptions of risk and uncertainty. Natalia Ospina-Alvarez from the University of Potsdam emphasized animations as a communication tool. They started a pilot project to develop teaching and learning material related to emerging contaminants that aims at the general public.
Marlea Wagelmans of Bioclear Earth contributed an example of how risk communication can and is actually carried out. She gave insight into risk communication of a water company in the Netherlands. They focused on the question: What to do when emerging contaminants are found in soil or water? They developed a roadmap for policy or actions by the province, drinking water companies or municipalities for emerging contaminants in the soil-water system. The roadmap describes the role of different stakeholders in this scenario including the role of communication.
Finally, Kees van Gestel from the Urise University Amsterdam introduced the initiative to develop an open online textbook about environmental toxicology that can be used for the training of B.Sc., M.Sc. and higher levels. By this, they aim at improving the quality, continuity and transparency of the education in environmental toxicology. They are searching for contributions from as many colleagues as possible from within the SETAC community.
A large crowd gathered around the presenters at the poster corner. While nearly no discussion took place during the session, many people from the audience used the availability of the presenters after the session to go into detail on certain aspects of their posters. The overall conclusion we could draw from the session is that many different tools and approaches can lead to an increase and improvement of science and risk communication. Social media, websites, online surveys and the online text book indicate that especially digital content are nowadays a proper means to reach out to target audiences. Most important is to structure any communication effort in a participatory way so that people can work towards their understanding of a scientific issue rather than consume information provided by researchers. All authors believed it’s worth it and plan to continue their efforts to promote these ideas.
The session was the seventh iteration in a series of sessions on science and risk communication at SETAC Europe annual meetings since the 2012 SETAC World Congress in Berlin, and it was sponsored by the SETAC Science and Risk Communication Interest Group – SCIRIC. It will hopefully find its continuation in the next session on science and risk communication at the SETAC Europe 29th Annual Meeting in May 2019 in Helsinki. Findings from this session are also valuable for the work of the SCIRIC IG towards tailor-made science and risk communication tools and strategies. They will help in developing a science-based risk communication plan for SETAC Europe, which is one part of the SETAC Europe strategic goals through 2020.
Summaries Published in the October 2018 Issue
Sebastian Höss, Ecossa (Ecological Sediment and Soil Assessment); Ute Feiler, German Federal Institute of Hydrology; Daniel Faber, Bayer AG, Crop Science Division; and Paul Sibley, University of Guelph
Healthy sediments provide a habitat for a diverse range of organisms whose interactions ensure the maintenance of important ecosystem functions, such as biochemical transformations, and thereby help to deliver unique ecosystem services, such as clean water. Therefore, they play a key role for the ecological status of aquatic ecosystems. On the other hand, sediments represent both a major sink and a potential source of persistent toxic substances in aquatic environments. Complex biochemical and ecological interactions occurring within sediments require specific assessment tools for protecting benthic organisms and processes for environmental risk assessment. Harmonization and improvement of sediment testing approaches is therefore a major goal for both prospective and retrospective risk assessment (figure 1).
This session was well attended, with a lively discussion and exchange of information and ideas, demonstrating the SETAC community continues to have high interest in environmental risk assessment in sediments. An important theme that emerged from the discussion was general recognition that sediment risk assessment should gain more attention in the global ecotoxicology community, especially within SETAC, and that SETAC’s Sediment Interest Group (SEDIG) could play a key role in facilitating this discussion.
In total, six presentations were included in this session and contained marine and freshwater case studies. This session was structured into two parts, retrospective sediment risk assessment and prospective sediment risk assessment.
Retrospective Sediment Risk Assessment
Starting this session, Susanne Heise, University of Applied Sciences Hamburg, reported on their study where they used a multiple line of evidence approach to assess the risk from historic contaminants in sediments of the Elbe flood plain. They could best describe the risk if combining chemical, ecotoxicological (toxicity tests) and hydrological (thickness of sediment layer, sediment transport) data for the risk assessment.
Elvio D. Amato, University of Antwerp, reported on experiences with an innovative passive sampling method using the diffusive gradient in thin films (DGT) technique as a tool to estimate the bioavailable fraction of metals in sediment. They found a good correlation of toxicity and bioaccumulation data derived with whole sediment exposure with DGT-estimated bioavailable concentrations.
Morten Thorne Schaaning, Norwegian Institute for Water Research (NIVA), presented a marine case study on the effects of mine tailing deposits on benthic fauna using a multiple lines of evidence approach. Although the benthic fauna still indicated good ecological condition at the sites with deposits, they concluded that both the current discharge to the water column and the leaching of copper and nickel from the sea deposits are likely to contribute to the moderate reduction of benthic biodiversity at the old deposit sites.
Prospective Sediment Risk Assessment
The start of the prospective risk assessment part of the session was introduced by Teresa Norberg-King, U.S. Environmental Protection Agency (USEPA). The presentation provided a good overview of refinements and improvements of the USEPA sediment toxicity test methods and protocols. This overview clearly demonstrated the existing differences between European and US sediment risk assessment methods.
During the presentation of Ivo Roessink, University of Wageningen, the differences in the use of artificial and natural sediment in a standardized test method was investigated. The results gave a hint that the tiered approach for the sediment risk assessment seems to work, but differences in sensitivity of the test organisms exists in the different sediment types, requiring a careful interpretation of study endpoints.
The last presentation of this session, given by Klaus Hammel, Bayer AG, focused on the spatial–temporal behavior of plant protection products (PPP) in the OECD standardized test guideline 219. It was demonstrated via modeled and measured data that the exposure in this test is driven by the physio–chemical characteristics of the PPP and the behavior of the test organism. Therefore, an interpretation of study endpoints for the risk assessment must be linked to the test organism and the test system used in the study.
Conclusions from the Session
The risk assessment of chemicals in sediments, both retrospective and prospective, is still an urgent topic in global environmental risk assessment as sediment contamination is often the main obstacle for reaching good ecological status in marine and inland waters. The presentations in this session showed that weight-of-evidence approaches using innovative techniques are necessary to explain ecological consequences and to unravel cause–effect relationships with chemical contamination. If the aim of a global harmonized sediment risk assessment approach should be fulfilled, a better understanding of the differences between U.S. and European risk assessment needs to be discussed and improved to become more harmonized. Furthermore, it was demonstrated that the interpretation of study endpoints from sediment studies needs a full picture interpretation of the given differences in the living behavior and exposure pathways of test organisms used for these studies.
Richard K. Cross, University of Exeter; Bitragunta Siva Prasad, Environment Protection Training and Research Institute; and Sankar Ganesh Palani, Birla Institute of Technology and Science, Pilani
The production of engineered nanomaterials is a rapidly expanding industry. Characteristics such as size, shape and surface functionality of engineered nanomaterials can be tailored during their production to confer properties distinct from the materials’ bulk counterparts. However, it is this increased reactivity of these materials at the nanoscale, generally defined as having at least one dimension less than 100 nm in size, which has stimulated research into the risk engineered nanoparticles (ENPs) pose to the environment during their lifecycle – from production to disposal.
As a research community, it is important to elucidate the role of properties intrinsic to ENPs that are responsible for their toxicity. In particular, screening tests and methodologies are needed to assess the potential for ENPs to interact with various components of environment, including other contaminants that could enhance their ability to cross cell membranes, thereby increasing toxicity in living systems. Currently, there is a paucity of data in this regard. Hence, understanding the multitude of possible interactions between ENPs, the abiotic environment and other contaminants is essential. Moreover, it requires communication and collaboration across a range of disciplines.
The aim of this session was to provide a space for that dialogue and bring together environmental chemists, nano- and ecotoxicologists, and regulatory scientists to present new findings that link and bridge these disparate fields. This session attracted keen interest and featured 26 posters alongside the five platform presentations. The talks identified potential tools to assess the mechanisms of toxicity of different NPs.
Aldo Viarengo, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Italy, began the session with a detailed assessment of the role of the mTOR pathway on physiological effects of fullerene C60nanoparticles in the digestive gland of the mussel Mytilus galloprovincialis. Viarengo demonstrated the application of chemical, immunohistochemical and transcriptomic analysis in combination to identify accumulation, subcellular distribution and physiological effects as a result of C60exposure. The findings indicated that changes in the phosphorylation of mTORC1 and mTORC2 could explain many of the effects observed in response to C60 at a cellular and tissue level.
Building on this theme and the application of -omics in the study of nanotoxicity, Diana Barros, Universidade do Minho, Portugal, presented a study on the proteomic responses to nanoparticulate and ionic silver in freshwater microbes from two sources: non-polluted or metal-polluted streams. Using this high-throughput analysis allowed differences in response to silver NPs and ionic silver to be detected. Interestingly, while both forms of silver induced stress-responsive proteins consistent with the response seen for enzymes involved in oxidative stress, results indicated that the biological pathways involved in exposure to ionic and nanoparticulate silver differed in the fungal strains tested.
The need for the development and application of screening tests to assess the toxicity of ENPs representative of the range of materials currently under production was argued by both Frida Book, University of Gothenburg, Sweden, and Sankar Ganesh, Birla Institute of Technology and Science, Pilani, Hyderabad Campus, India.
Book presented a case study of the cellular toxicity of silica NPs using a battery of tests to investigate their toxicity at a range of biological levels. Fish gill cell lines were found to be the most sensitive to the panel of seven silica particles assessed in this study, with toxicity being size dependant. The smallest particles (7 nm) were the most toxic to the gill cell lines, inducing effects in the low mgL-1 concentration range. The experimental design allowed different exposure metrics to be compared against the observed toxicity (mass concentration, number concentration and surface area-based exposures). This assessment considered surface area as the best explanatory characteristic for the observed toxicity.
Ganesh presented an application of the OECD-207 test guideline exposing the terrestrial earthworm Eisenia fetida to rutile titanium dioxide ENPs (r-TiO2-NP). No mortality was observed at concentrations up to 0.25 mgkg-1TiO2, however, sub-lethal endpoints were sensitive to these concentrations. The case was presented for using activities of antioxidant enzymes including catalase, superoxide dismutase and glutathione reductase as biomarkers of oxidative stress in this sentinel terrestrial species.
The session was closed with Željka Lončarić, University of Osijek, Croatia, using biomarkers and reproductive success as indicators of toxicity of mixtures of zinc oxide nanoparticles (ZnO NPs) and the pesticide chlorpyriphos to the earthworm Dendrobaena veneta. Studies were performed in both artificial and natural soils, and effects were assessed between generations of the exposed earthworms. Key findings included that mixtures were more toxic in artificial soil than the natural soil during these exposures, potentially due to changes in behavior of the contaminants under different soil conditions. Nano ZnO particles were also found to elicit greater toxic responses than bulk Zn during these exposures.
In conclusion, this session identified several emerging techniques and methods for assessing the complex interactions between engineered nanomaterials and biota, which could be used to elucidate the mechanism and mode of action of nanomaterials in the environment. High-throughput techniques are required to generate the data necessary to identify characteristics of nanomaterials that determine their toxicity. In particular, proteomics was demonstrated as a useful tool to provide mechanistic insight into the stress induced by nanomaterials, with particular application for distinguishing between different mechanisms of action involved in nanoparticle and their corresponding ionic exposures.
The case for using multiple techniques to understand the mechanisms of toxicity during nanoparticle exposures was made in each presentation. Successful application of techniques and approaches varying from the physicochemical characterisation of the ENP, assessment of biochemical markers, immunohistochemical staining and -omics were demonstrated by the various speakers.
A suite of biochemical markers were proposed for assessing sub-lethal effects of engineered nanomaterials, including antioxidant enzymes (catalase, superoxide dismutase andglutathione reductase), lipid peroxidation, glutathione S-transferase, metallothionein and AChE. Use of these biomarkers for the assessment of toxicity to TiO2-NPs and mixture effects of ZnO and pesticides has been presented.
Surface area was found to be the best explanatory metric for the toxicity of silica NPs, and the general discussion within the session concluded that careful consideration of exposure metrics will allow future work to better interpret toxicity in the context of nanoparticle properties.
The topics raised during this session outline a focus for future research, while the poster session conducted in parallel to these talks provided space for researchers to communicate directly with one another. This facilitated the sharing of technical details and methods under development in this field.
Author’s contact information: email@example.com
Fabienne Ericher, Cambridge Environmental Assessments; Jason Weeks, Joint Nature Conservation Committee; Anja Kehrer, Umweltbundesamt; Jaana Laitinen, European Chemicals Agency
The session covered a variety of subjects with relevance to both sectors, the biocides and the veterinary medicine.
The key points of the six presentations included:
- Jason Weeks of the Joint Nature Conservation Committee introduced regulatory updates for veterinary medicine and presented the on-going work of the Environmental Risk Assessment Working Party (ERA-WP) of the committee for veterinary medicine products (CVMP)
- Ricardo Carapeto Garcia, Agencia Española de Medicamentos y Productos Sanitorios, facilitated discussion on how operational protection goals could be defined for the risk assessment of plants, in the context of veterinary medicines scenarios and helping reduce uncertainties, defining risk mitigation measures and improving the benefit–risk balance
- Jane Staveley, Exponent, gave a presentation on an innovative environmental risk assessment of an endocrine-active substance, including field-scale (watershed-level) modeling, in the context of a U.S. registration of a veterinary medicine
- Laszlo Doren, ERM, discussed how mesocosms studies may be used to provide additional ecotoxicological information and support biocides and vetmed registration, covering the key points of planning, carrying out and evaluating these type of studies
- Rikke Gleerup Ovesen from the Danish Environmental Protection Agency provided an example of successful scenario design addressing a biocidal product used for veterinary hygiene, which sits in the gray area between the two sectors. This is also a project that was brought to completion following cooperation between the industry and regulators
- Stefanie Wieck, Leuphana University, looked at household emission sources of active substances, which highlighted how a significant share of biocidal active substance found in the environment may actually come from uses covered under other regulations
The poster presentations expanded on the subjects covered in the platform presentations, with several posters discussing monitoring projects, emission scenario developments and methods. In addition, several posters addressed themes not discussed in the platform presentations, including leaching from façades and strategies to reduce workload during the dossier preparation process.
In conclusion, the presentations touched onto a wide range of topics, from hazard assessment and exposure scenario design to environmental monitoring and risk management aspects. Many of the issues were of relevance to both sectors (e.g., protection goals, higher-tier aquatic studies, endocrine-active substance) and there was reference to products that sit in the borderline area between biocides and veterinary medicine. This session highlighted that current challenges are often shared across sectors but also that the solutions developed have some relevance across sectors. There is great potential to be gained by keeping in touch with other sectors’ developments and newly designed approaches. SETAC Europe is an opportunity for professionals from the biocides and veterinary medicines area to share developments and to gain from the technical advances acquired in the represented sectors.
Andrea Porcari, Italian Association for Industrial Research, and Andreas Ciroth, GreenDelta
Assessing social impacts of products is gaining more and more importance in policy and business strategies, and Social Life Cycle Assessment (S-LCA) provides a methodological approach to this end. The recent notion of Responsible Research and Innovation (RRI) underlines the advantages of early assessment of impacts, starting from agenda setting and research and development (R&D) phases of product development.
S-LCA and RRI could provide valuable approaches to assess social performances of organizations (companies in particular), better align products with societal needs, and work toward sustainable development goals. Stakeholders concerned include workers, local communities, consumers, and value chain and supply chain actors, as well as the broader society. Both positive and negative impacts are taken into consideration. However, practical implementation of S-LCA and RRI is still limited, and there is a need to develop practical case studies to collect further experience and improve existing tools and methods.
The purpose of this session was to illustrate experiences on S-LCA and RRI from both the industrial and policy areas, and explore connections between these two emerging approaches dealing with social impact assessment. The session covered views from different experts and stakeholders from both research and business, followed by an interactive plenary discussion.
The key points emerged from the five presentations and three spotlights included:
- Developments and recommendations on the practical use of S-LCA was presented by Silvia di Cesare, CIRAD, Italy. She underlined the increasing demand of companies for S-LCA as a tool to enhance their social performances and complement their sustainability reporting. Improving existing databases, further developing quantitative indicators and increasing the use of participatory approaches to define system boundaries were underlined among the key steps needed to improve existing S-LCA methods.
- The Prisma Project on piloting RRI in industry was presented by Andrea Porcari, Airi, Italy. S-LCA and RRI share the scope of performing social impact assessment of products, with the latter having a specific focus on R&D, and early stage intervention in the research and innovation value chain (anticipation of impacts). The H2020 Prisma project is using a case-by-case, expert-driven approach to integrate RRI in research and innovation projects of eight companies dealing with transformative (nanotech, Internet of Things, synthetic biology, autonomous vehicles), offering insights on the potential benefits of RRI for industry.
- Sabrina Neugebauer, RWTH Aachen University, Germany, presented on the social significance analysis of products, considering negative and positive social impacts along the supply chain of leather products. She provided a practical example on how S-LCA could help to identify and compare hot spots of social concerns (e.g., issues related to hours of work, health and safety, equal opportunities, social security and delocalization), across a wide range of countries in the leather supply chain. Qualitative and semi-qualitative indicators have been considered, partially when adapting the UNEP–S-LCA assessments.
- “Integration of Sustainability in Industrial Research and Innovation: Perspectives from ArcelorMittal’s Experience” was presented by Anne-Laure Hettinger, ArcelorMittal, France.
This very large industrial group is developing an extensive strategy (sustainable development framework) to improve the sustainability performances of their products, combining aspects related to Corporate Social Responsibility (CSR), S-LCA and RRI. The strategy aims to cover the very diverse set of technologies and products related to the steel sector and to impact researchers and employers of the company worldwide. Key aspects include an easy to use self-assessment tool for researchers, hot spot analysis of all research and innovation projects, analysis of added values of sustainability for specific products, materiality analysis and identification of indicators.
- Ivan Muñoz, 2.-0 LCA consultants, Denmark, presented on the social footprint of a packaging waste deposit–refund system in Spain. The assessment of the social footprint is based on a dedicated analysis to include correction factors to the gross domestic product and purchasing power, based on externalities related to environmental and social aspects obtained from a top-down analysis using “IO data” (e.g., household production, trade barriers, unemployment, avoidable health impact, underinvestment in education). Indicators were used to make an S-LCA analysis to evaluate positive and negative impacts of introducing a recycling process in packaging waste system on a local community.
Three spotlight presentations were also given:
- Applying Social-LCA and Social Hot Spot Analysis including a SDG Evaluation to Product Assessments with SEEBALANCE® from Peter Saling, BASF SE, Germany
- Sustainable Guar Initiative – An Integrated Approach of Social and Environmental LCA from Alain Wathelet, Solvay SA, Belgium
- Social Life Cycle Assessment of the Water System in Mexico City from the Engineering Institute Universidad Nacional Autónoma de México
Conclusions from the Session
S-LCA and RRI provide science-based methodologies to improve policy making and business strategies developments to help better address social needs and societal development goals (SDG).
As shown by practical cases studies, the quantitative approach of S-LCA could support decision-making in policy and business at global, regional and local levels. The availability and reliability of databases strongly influence the assessment. The use of a mix of qualitative and quantitative data, and comparative assessment of negative and positive impacts, facilitate different levels of analysis.
RRI could complement S-LCA, looking also at R&D and technology development activities. In addition, with the use of case studies, experiences of practical actions and operations at organizational level provide examples of early intervention to address social impacts (that can help improve the societal performances measured by S-LCA).
Further analysis and considerations of mutual benefits of S-LCA and RRI approaches is envisaged.
Séverine Le Faucheur, University of Geneva; Michael S. Bank, Norway Institute of Marine Research; João Canário, Técnico Lisboa; and Nelson J. O’Driscoll, Acadia University
Mercury (Hg) is a persistent pollutant, and emission, in some areas, have been increasing over the last decade due to anthropogenic activities such as gold mining, coal burning, chlor-alkali manufacturing and non-ferrous mining extraction. Gaseous elemental Hg can be transported far from its source and deposited to remote areas. When Hg enters aquatic and terrestrial systems, it undergoes several physical, chemical and biological reactions. Microbial methylation is one of these key processes and leads to the formation of methylmercury (MeHg), a potent neurotoxin. MeHg can biomagnify along the food chain, making top predators, such as fish and humans, susceptible to exposure of MeHg. The foundations of the Hg biogeochemical cycle are well studied; however, several aspects and questions remain unresolved and impede us to fully comprehend its behavior and impact on aquatic ecosystems and the organisms that inhabit these environments. The aim of our session was to illustrate those limitations and to identify important advances in environmental Hg research.
For the first platform presentation, Mae Gustin, University of Nevada, discussed the role of Hg speciation and Hg atmospheric chemistry, as well as the possible underestimation of gaseous oxidized Hg concentrations due to a lack of appropriate measurement techniques and instrumentation. She also pointed out that gaseous oxidized Hg exists under several forms as a function of oxidants present in the air. Two posters also focused on atmospheric Hg chemistry, one by Stefano Covelli, University of Trieste, and another by Alessandra Fino, Italy National Research Council, who presented work on monitoring gaseous Hg evasion fluxes in low- and high-impaired Hg sites including coastal areas.
The next few oral presentations focused on the potential mitigation effects of selenium (Se) on Hg bioaccumulation. Atabak Azad, Norway Institute of Marine Research, presented Se:Hg molar ratios in marine fish, which is the most important biotic compartment when addressing public health. His presentation discussed trends and rates of Hg bioaccumulation with regard to fish species, length, life history and geography within the Northeast Atlantic Ocean. Data analyses were conducted on more than 8,000 fish tissue samples and provided encouraging results on seafood safety and human consumption (less than 1% of sampled fish fillets were considered problematic). Jacqueline Gerson, Duke University, also discussed Hg and Se at a lower level of the food web in freshwater ecosystems. Her investigation demonstrated that Se reduced the percent of MeHg measured in sediment, biofilms, crane flies and spiders. In crane flies and spiders, that observation was also valid for the absolute MeHg concentration. Session chairperson Michael Bank, Norway Institute of Marine Research, gave an impromptu presentation on long-term temporal trends and Hg bioaccumulation regimes in circumpolar Greenland halibut. Bank presented a series of Bayesian uncertainty models demonstrating the role of time and food web complexity on MeHg dynamics in Greenland halibut in the context of climate change and biomonitoring efforts in support of the implementation of the UN Minamata Convention on Mercury.
Finally, Igor Zivkovic, Jožef Stefan Institute, talked about his latest research on the role of nutrients on the microbial formation of MeHg and dissolved gaseous Hg in the Central Adriatic Sea. His research successfully showed that MeHg was related to microbial parameters only when phosphate was not limited. In contrast, no clear biotic link could be observed with dissolved gaseous Hg. Perrine Dranguet, University of Geneva, presented a poster highlight that also focused on the role of microbes in the Hg cycle and showed the importance of biofilm microstructure and composition in Hg accumulation by biofilms.
The Hg session at SETAC Rome was a great success and garnered interest from approximately 100 audience members. The session demonstrated that the development of analytical tools for accurately measuring Hg in the atmosphere is still an active research area that needs to be addressed, especially due to the key role played by gaseous Hg in long-range transport. We also saw several examples demonstrating that ambient water chemistry and food web complexity are major drivers of Hg bioaccumulation in marine and freshwater fish and other biota. These findings are extremely important for the ongoing efforts of the Conference of Parties of the UN Minamata Convention on Mercury. Article 22 of the convention requires an “effectiveness evaluation,” partly based on the monitoring of Hg concentrations in abiotic and biotic compartments. Identifying important drivers of the atmospheric Hg cycle and bioaccumulation regimes in biota will help us to understand future environmental Hg concentrations and spatial–temporal trends in the context of a rapidly changing environment.
The next Hg session is planned for the SETAC Europe 29th Annual Meeting to be held from 26–30 May 2019 in Helsinki, Finland.
Gert Everaert, Flanders Marine Institute; Jörg Römbke, ECT Oekotoxikologie GmbH; and Martina G. Vijver, Leiden University
Chemical and ecological data are often collected under different research or monitoring programs. Merging different sets of data has significantly increased our ability to investigate and quantify how a multitude of stressors may potentially alter biodiversity. In those cases, many aspects need to be tackled, including:
- Missing data
- Consistency of data
- Coherence of the data
- Existence of reference sites
Due to the different aggregation and statistical methods, as well as increased computational power, we can get more information out of those datasets than what is currently the case. In this session, we recommended focusing on meta-analyses of existing datasets, with an aerial view on meta-analyses in scientific research in general and ecotoxicology more specifically.
The session was opened by building further on one of the pending questions at the end of a similar session in 2017 at SETAC Brussels: “What are big data?” It was argued that big data is a buzzword and that for the broad audience, but also for fellow scientists, big data often relates to -omics data in which whole genomes are sequenced, social media data that we spread via our mobile computer devices, or industrial processes in which big data analytics are used for commercial purposes, to quality check, reveal patterns from or even improve processes.
In our opinion, big data has certainly its place in eco(toxico)logy. Examples are the continuous monitoring of water and temperature levels or observations of ecotoxicological experiments by means of a flow cytometer or high-throughput methods. Also, in terms of collecting existing data, big data applies to ecotoxicology and applied ecology, some examples given in this session were EDAPHOBASE, the REACH database and the USEPA ECOTOX database. These databases can be used to answer long-lasting questions in various environmental regulations, such as the assessment of contaminated land.
We started the session with a talk from Martina Ross-Nickoll, RWTH Aachen University, who presented on the EDAPHOBASE soil diversity warehouse. This talk illustrated how data are collected in a soil-zoological information system and how it combined existing taxonomical primary data on soil organisms from collections, scientific literature and reports. In addition to collecting data, EDAPHOSTAT is the very first interactive web application that provides data exploration. The future intention of EDAPHOBASE is to expand towards a pan-European soil-biology data warehouse, providing a reference base of the ecological quality of soils as described by structural and functional data of soil organism communities.
Once the data are collected and first steps in data exploration have been performed, Erwan Saouter, EU Commission JRC, showed us how to use the big datasets in the context of the EU Commission Product Environmental Footprint (PEF) activities. The potential impact of chemicals emitted during the life cycle of a product is assessed via the USEtox multimedia fate model. For each single chemical, this model requires dozens of physico–chemical parameters, as well as data on ecotoxicity to freshwater aquatic life and toxicity to human for cancer and non-cancer endpoints. In his talk, Saouter illustrated how user-friendly R is thanks to its free access, free packages, clear guidance documents and responsive community. In his work, all REACH registration data were used to calculate chemical effect factors.
Sanne Van den Berg, Wageningen University, guided us through the technical decisions taken at the start or during modeling processes. These decisions can impact the ultimate trends and patterns that are inferred from the data. She showed us what the impact is of modeling decisions made while or at the start of different modeling approaches. These impacts of decisions were compared against each other by using cross-validation errors by using adjusted R squares and Akaike Information Criterion. For the eco(toxico)logical modeling applied by van den Berg it was found that 1. using AIC as model selection criterion results in models that are better in predicting sensitivity outside the training data, 2. species-traits matching should ideally be done at the genus level, 3. the method chosen for trait predictors depends on the envisioned modeling purpose (mechanistic understanding or improved predictions), and finally, 4. that the collinearity threshold greatly determines the reliability of the resulting models. If we want to look into the future, images and image recognition will become increasingly important in ecotoxicological research.
In this context, Sizandreu Abreu, University of Aveiro, illustrated a D counter to the audience, which is a counter that can be used for image recognition and automatic counting of test organisms. This device is one of the tools considered to produce a lot of data in future. In the final platform presentation of this session, Kristin Connors, Proctor & Gamble Company, argued that both Daphnia magna and Ceriodaphnia dubia should be fully accepted for regulatory toxicity requirements based on a meta-analysis of existing data that given their shared life history, geographic range, and acute and chronic sensitivity. This has huge consequences for regulatory documents because it can result in the fact that multiple species can now be used in toxicity tests, and this way, the number of ecotoxicity data can be extended hugely. We ended this session with three poster spotlights that illustrated some hands-on applications of big data in ecotoxicology, i.e., on the “holistic” assessment of a huge data set from standard earthworm field studies, presented by Frank Staab, BASF SE; the use of historical control data from aquatic mesocosm studies to improve the interpretation of results from individual studies, presented by Hanna Schuster, Cambridge Environmental Assessments; and new tools for data visualization and database interoperability using the USEPA database ECOTOX as an example presented by Kellie Fay, CSRA.
Given the increasing amount of data generated under (inter)national monitoring programs, in research projects and under laboratory conditions, and in order to have these data available for meta-analyses, we should respect the FAIR data principles on Findable, Accessible, Interoperable and Reusable data. Only by doing so, data can be found by fellow scientists for meta-analyses and can be used to answer long-lasting environmental questions.
Summaries Published in the September 2018 Issue
Francisca Fernandez-Piñas and Miguel Tamayo Belda, Autonomous University of Madrid, and Roberto Rosal, University of Alcalá
Microplastics have long been recognized as environmental pollutants causing effects to marine and terrestrial life. However, the importance of the smaller fractions, nanoplastics, has only been documented in recent times. Nanoplastics, like other nanomaterials, have unique properties due to their size, shape and their capacity to interact with other substances due to their large surface area. Nanoplastics can produce physical damage, but they may also be transported across cell membranes and persist in the environment due to their relative inertness. In this regard, mechanistic approaches are needed in order to identify the cascade of signaling events at different levels of biological organization, which induce a cellular, whole organism or ecological response to micro and nanoplastic exposure.
Plastic materials can act as vectors of environmental contaminants or can cause an indirect toxic effect due to the release of the additives they contain, such as flame retardants or plasticizers, which, on average, represent about 4% of the weight of plastic materials. These additives may impose an additional risk factor for which very little is known.
Microorganisms may get adsorbed onto the plastics and form stable biofilms; in this context, plastics materials may also become a vector or reservoir for the spreading of potential pathogens or antibiotic resistance genes at a global scale.
The main purpose of the session was to gather the information required to evaluate the risk posed by micro- and nanoplastics to the environment and human health in real-world scenarios. In this context, reports on mechanistic approaches to study the biological action of plastic materials on organisms at different trophic levels and those on the role of micro- and nanoplastics as vectors for other pollutants and also as habitats for microbial communities were particularly welcome.
The session attracted high interest; initially 41 abstracts were submitted, 15 of them to be considered as platform presentations. Ultimately, five platform presentations and three poster spotlights were delivered in the session. These were selected in order to cover as much as possible of the main goals of the session.
The first presentation by Shima Ziajahromi, Griffith University, addressed the presence of microplastics in wastewaters with a novel validated sampler, finding that wastewater treatment plants (WWTPs) play an important role in releasing microplastics to the receiving bodies. The study showed there was a dose-dependent effect of real environmental concentrations of microplastics on Ceriodaphnia dubia and the sediment dwelling larvae of Chironomus tepperi. The second platform presentation by Lisa Zimmermann, Goethe University Frankfurt am Main, presented a thorough study on the biological effect of additives already present in daily-use plastics; extracts from 34 plastic consumer products were tested for unspecific and specific toxicity using different whole cell bioreporters, demonstrating that the intrinsic plastic additives, when released, may be toxic for the biota. The third presentation by Chang Bum Jeong, Sungkyunkwan University, reported a thorough mechanistic approach to understand the toxicity of a range of sizes of polystyrene microbeads in a rotifer. The fourth presentation by Bettie Cormier, EPOC University of Bordeaux, and the fifth presentation by Leticia Vidal-Liñán, University of Vigo, dealt with microplastics as vectors for pollutants. The former focused on toxicity derived from the sorption of model pollutants on microplastics using embryos of zebrafish, which demonstrated that microplastics may act as vectors of persistent organic pollutants, particularly PFOS, but did not find any associated toxicity for the embryos. The latter presented a comparative study of the role of microalgae and microplastics as vectors of the organophosphorus insecticide chlorpyrifos and the subsequent effect on marine mussels. The study found a similar effect for both vectors, which was clearly different from waterborne exposure of the insecticide.
Thirty-two posters were submitted to the session. Among these, three were presented as poster spotlights. Salla Selonen, University of Helsinki, presented the first poster spotlight, which dealt with the potential effect of synthetic textile fibers on soil organisms as these fibers may end up in agricultural soils causing slight negative effects on soil-dwelling worms. Migel Pleiter, Autonomous University of Madrid, presented the second poster spotlight and reported that the abiotic depolymerisation of the biodegradable plastic PHB, usually regarded as safe, resulted in nanoparticles that were toxic for phytoplankton. Messika Revel, Catholic University of the West | UCO, presented the third poster spotlight. She studied the bioaccumulation and toxicity of two types of microplastics in blue mussel, Mytilus edulis, at environmentally relevant concentrations, where it was found that microplastics were only present in the digestive glands of the mussel and only at the highest concentrations tested. The findings reported potential oxidative stress in the organism.
The poster session was very interesting with studies covering different aspects of detection, bioaccumulation and toxicology of micro- and nanoplastics in a myriad of cells and organisms of environmental relevance: phytoplankton, Daphnia, bivalves, crustaceans, fishes, nematodes, echinoderms and polychaetes. Several posters addressed thorough mechanistic studies – biochemical and molecular – on the biological effect of micro- and nanoplastics, while others focused on ingestion and bioaccumulation. Several dealt with plastics as vectors of pollutants and additives, studying kinetics of pollutant sorption on the plastics and effects of mixture toxicity. Three posters generated attention as they dealt with contamination and effect of plastics in polar regions. Finally, two posters openly discussed the true impact for both environmental and human health of the presence of microplastics in different environmental compartments, including food and beverages. Unfortunately, not a single study on plastics as vectors of microbial communities was presented.
The session was very successful and the congress room was completely full, with many people standing up. The discussion was fruitful and dynamic with intense debate.
In conclusion, the presented works tried to address the question of whether micro- and nanoplastics may pose a threat to environmental and human health:
- Some works demonstrated that these kinds of materials may be harmful for some organisms of ecological relevance, while in other studies it was not so clear that micro- and nanoplastics could be considered as toxic.
- Toxicological studies should be made at environmentally relevant concentrations, though that is difficult to determine for the smallest fraction, nanoplastics. Although, higher concentrations could be needed to determine mechanistic mode of action on most model organisms.
- Plastic may behave as vectors of adsorbed pollutants, but more studies are needed in order to find out which have toxicological consequences.
- Bioaccumulation might be an issue, but further studies are also required, in particular, for those organisms that may ingest but also egest the plastics.
- Long-term studies are needed to fully understand the effect of micro- and nanoplastics on the biota in different environmental compartments.
For the general public, the accumulation of plastics in water, particularly in the oceans, is becoming a serious concern. No one can deny that they are an important factor when considering environmental pollution. But besides the aesthetic issue, there is a need for further multidisciplinary research to get insights into the true impact of these materials in nature.
Hans Chritian Bruun Hansen, University of Copenhagen, and Gemma Gimémez Papiol, Fondazione IMC – Centro Marino Internazionale Onlus
The session comprised 12 talks and 36 posters with a focus on natural toxins produced by aquatic micro-organisms or terrestrial plants with implications for food and drinking water quality and safety. The session, which was attended by more than 150 people, gave new insight with advances in analysis, monitoring, distribution, toxicity and handling of risks of both known and new classes of cyanobacterial toxins, palytoxins, geosmin and emerging natural toxins from plants such as terpenoids and saponins. Many of these are highly toxic, may be produced in substantial amounts and, thus, present serious threats to food and water quality. However, the challenges faced with these natural toxins are complicated due to the very high number of toxins, their structural diversity, biological controls of production and diverse modes of action. There is a strong need to develop sensitive analytical methods to enable monitoring and to combine with fate processes and toxicology for proper risk assessments. Toxin production is linked to anthropogenic perturbations, such as eutrophication and monocultures, and also the climate dimension is apparent through influence on the spread of toxin-producing, sometimes invasive, organisms, toxin production and forms of toxic producers. The talks may be arranged into the following five groups:
- Cyanotoxin analysis
- Biological interactions
Anastasis Hiskia , National Center for Scientific Research “Demokritos,” gave an overview of sample preparation and Liquid Chromatography Mass Spectrometry (LCMS) methods used for cyanotoxins in Greek lakes emphasizing the need for selective and sensitive methods. Samples were measured for microcystin and nodularin peptidic toxins, as well as cylindrospermopsins, anatoxins and saxitoxins. Microscysins were not dominating in the lakes, and with total concentrations reaching mg/L levels.
Regiane S. Natumi, Swiss Federal Institute of Aquatic Science and Technology, gave an overview of peptide toxin classes produced by cyanobacteria and sensitive analytical methods for these toxins. She demonstrated that different species produce toxins belonging to different classes and that nitrogen and phosphorus deficiency may stimulate toxin production.
Klara Hilscherova, Masaryk University, presented a new class of retinoid (vitamin A-like) compounds produced by cyanobacteria. These compounds are important in signaling and regulating cell processes and may produce theratogenic effects in exposed organisms. Hilscherova gave examples on effects in zebrafish and frog embryos, and the compounds shown to be present at concentration levels in field samples able to produce theratogenic effects.
Claudia Wiegand, University of Rennes 1, presented a lab study to show that Daphnia magna (representative of zoo-plankton) – although being harmfully affected by grazing on cyanobacteria – also has reverse effects on these, causing an increase in antioxidant activity. Thus, cyanobacteria and zooplankton interact not just one way.
Finally, Maura Manganelli, Istituto Superiore di Sanità, presented data for cyanotoxin adverse effects on humans and presented threshold values to protect humans during bathing via either oral, dermal or inhalation exposure with focus on microcystins, anatoxins and cylindrospermopsins. Manganelli found it was important to both measure microbial cell and toxin concentrations.
High-Throughput Methods for Monitoring of Cyanotoxins
James Lazorchak, U.S. Environmental Protection Agency, presented a new method to determine total microcystins in fish tissue. The method has been tested for different fish species spiked with the toxins. First field measurements show that the method needs further improvement.
Sergio Bodini, SYSTEA S.p.A., presented an automated immunoaffinity-based method for determining three algal toxins in seawater. A portable unit has been developed, which allows for online determination; first testing has shown successful; inclusion of a preconcentration step may improve sensitivity.
Other Water-Born Toxins
Carmella Dell’Aversano, University of Naples Federico II, gave an overview of palytoxins (including ovatoxins) produced by Ostreopsis spp. (dinoflagellate) and with examples of Liquid Chromatography High Resolution Mass Spectrometry (LCHRMS) and Nuclear Magnetic Resonance (NMR) structural elucidation and toxicity of this class of non-peptidic toxins. Toxin monitoring along the Mediterranean coasts have spotted regions with high polytoxin levels covering different toxic profiles. Dell’Aversano emphasized the huge need for reference materials, fate and toxicity studies.
Carmen Espinosa, University of Vic – Central University of Catalonia, presented a temporal monitoring study of geosmin toxins in the Ter catchment in Spain. Geosmin produced by cyanobacteria and actinomycetes were generally produced above the human detection level of 5–10 ng/L and showed to increase with growing eutrophication but were also affected by nitrogen/phosphorus imbalances.
Plant Toxins as Water Pollutants
Lars H. Rasmussen, Metropolitan University College, gave an overview of carcinogenic toxins (ptaquilosides) produced by Bracken – their global presence, mobility, degradation (hydrolysis) and occurrence in surface and groundwaters. He elucidated how geographical, biological, climatic and temporal factors may control bracken toxin pulses in water reservoirs.
Nina Cedergreen, University of Copenhagen, highlighted species sensitivity towards saponin toxins, of which some are being used as biopesticides. She emphasized that toxicity cannot be read across but depends on both saponin structure and the species exposed. Examples were given of apparent high stability of Quillaja saponins in lake water.
Climate and Food Safety
Angelo Maggiore presented European Food Safety Authority (EFSA) work with early awareness and screening of how climate effects food safety. He presented the climate change and emerging risks for food safety project (CLEFSA) and gave examples of how natural toxin contamination (such as ciguatoxins, cyanotoxins, tetrodotoxins and BMAA) may be affected by climate. He asked for further input and stretched the importance of communicating the emerging risks to the public.
The environmental chemistry and toxicology of natural toxins is developing as a field, with much still to be learned. There is a strong need for development of robust sample preparation and analytical methods, and for studies of fate, mobility and toxicity, to lay the basis for proper risk assessment, actions and communication. Analysis of the toxins suffer from lack of standards and reference materials, as well as availability of radioactive labeled substances. In particular, there is a serious lack of monitoring data for non-cyanobacterial toxins. Controls on toxin production and ecosystem effects are complex and need further elucidation.
The natural toxin group started with about 25 attendants and a poster corner at SETAC Brussels in 2017, but this year it has grown to a much larger group, with almost 50 contributions. It demonstrates that this group of emerging contaminants is of growing interest, and we look forward to adding new data and insight next year at the SETAC Europe 29th Annual Meeting, which will be held from 26–30 May 2019 in Helsinki, Finland.
Anna Barra Caracciolo, Paola Grenni and Luisa Patrolecco, Italian National Research Council – Water Research Institute, and Donato Ludovici, Italferr S.p.A.
In the coming years, due to the numerous tunnelling projects planned in Europe and worldwide, several hundreds of millions of tons of spoil material will be produced. Tunnel debris is composed of soil and rock treated during excavation processes with different chemical products (e.g., foaming agents and polymers) used for soil conditioning. While tunnel debris can be used for many purposes, three potential uses of tunnel debris are refilling old quarries, road construction material and as a raw material for industrial production. Commercial foaming products are chemical mixtures that can include multiple compounds. The persistence and ecotoxicity of these chemicals can influence the subsequent possible reuse of the huge amount of soil debris as byproducts or its discharge as waste. There are currently neither soil threshold limits in European or Italian legislation for several chemicals present in the chemical formula of soil conditioning additives, nor comprehensive studies on its ecotoxicological effects on soil and water organisms. The development of modern and efficient high-speed railway and motorway networks requires a responsible innovative technology and common criteria to assess and manage the possible environmental risk of the residual mixture of chemical occurring in the spoiled material.
The session “Environmental risk assessment and management of the spoil material produced in tunnelling excavation” took place at the SETAC Europe 28th Annual Meeting in Rome and was aimed at promoting an interdisciplinary discussion among chemical producers, engineering contractors, stakeholders (e.g., railway and motorway operators), environmental scientists and regulators on the complex topic of management of the spoil materials obtained from excavation performed with tunnel boring machines (TBM).
In fact, this topic was discussed in this session from different points of view, such as using low-impact commercial products, evaluating common criteria for the environmental risk assessment of spoil material, taking into consideration different possible exposure scenarios and proposing new European guidelines or regulations in order to make the environmental protective goals uniform between the various countries. Six oral presentations (including case studies) from stakeholders and researchers from academia, chemical producers and engineering companies were delivered. Additionally, 10 posters in the dedicated poster session were presented. Please reference the SETAC Europe 28th Annual Meeting abstract book for a full list of presenters and details of their talks.
Jessica Legradi, VU Amsterdam; Riccardo Massei, UFZ – Helmholtz Centre for Environmental Research; and Jorke Kamstra, Norwegian University of Life Sciences
Fish model species are widely applied in human and environmental toxicity studies, since they present several advantages in respect to other model organisms. As major examples, fish species cover a wide range of habitats (from salt to fresh water), types of reproductions (from livebearers to egg-laying species) and size (species that are only a view centimeter in size and others that are up to several meters). It is clear the heterogeneous characteristics of each species allow their application in disparate research fields, ranging from human medicine and disease etiology to ecotoxicological studies. Therefore, the proper selection of a fish model can improve the scientific outcome and relevance of toxicity studies. In this context, one of the major goals of the session “Fish model species in human and environmental toxicology” was to bring together the latest scientific outcomes in toxicological studies using fish species and to create a link between international scientists from different research fields. As result, the session showed an outstanding participation characterized by a great interest throughout the platform presentations and poster sessions.
The first part of the session was dedicated to research performed with zebrafish (Danio rerio). Within the first session, new state of the art technologies were applied with zebrafish to gain deeper insights into specific toxicological mechanisms. The first impression was that the days of simple dose–response curves and LC50studies are over. In fact, many scientists are now trying to achieve a deeper understanding of specific molecular processes, in particular an understanding of the metabolic capacities of zebrafish during adult and embryonic stages.
As an example, the effects of environmental levels of selenium on energy metabolism were shown. Swimming performance was assessed via a “fish treadmill,” a tube where fish must swim against a constant current. Energy metabolism was monitored by linking resting and swimming performance with oxygen consumption. Moreover, the heart function was analyzed measuring cardiac output using ultrasound bio-microscopy. These results suggested that aquatic selenium pollution might induce a “metabolic syndrome,” thus acting as an “obesogenic” and an “endocrine disrupting” compound. Especially relevant for zebrafish studies using embryonic stages was a presentation about the metabolic capability at different developmental stages. A high capability and capacity of the zebrafish embryos with respect to the biotransformation of xenobiotics was found after 24 hours of development. An interesting applicability for zebrafish embryo toxicity testing was the use for particulate matter of different particle sizes. Still in the field of -omics, new insights into sex- and organ-specific estrogenic effects using non-targeted proteomics were found using High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS). Such studies might help find candidate molecular toxicity biomarkers for early prediction of apical endpoints such as reproduction. Finally, a couple of studies argued for the use of zebrafish embryos as potential monitoring and diagnostic tools in environmental studies. Zebrafish embryos were also presented as a diagnostic whole mixture assessment tool. Toxicological profiles were established after direct sediment exposure. Site differences for locomotor responses, malformations, acetylcholinesterase activity and heartbeats were shown.
In the second half of the session, the focus switched from zebrafish to other widely used fish species. Also in this case, the presentations confirmed the growing interest of scientists for new molecular techniques and their possible application in toxicology and ecotoxicology. The first presentation focused on dietary effects of methylmercury of the fathead minnow (Pimephales promelas) and the house mouse (Mus musculus). Effects on behavior, growth, immune function, metabolism or energetics, neuroendocrine function and central nervous system structure were reported. Similar effects were observed in mice, highlighting the strong correlation between fish and mammalian species. It was suggested the gut microbiome might be a key player in directly or indirectly regulating these effects. In the next presentation, the effects of Fluoxetin (a widely used antidepressant) on rainbow trout (Oncorhynchus mykiss) and white sturgeon (Acipenser transmontanus) using RNA sequencing were presented. The two-test species showed very different transcriptional responses, which might be due to metabolic or uptake differences. Neurotoxic effects could be seen but not the suspected response on anxiolytic genes. This might indicate that fish might respond differently than humans to such substances.
Transgenerational effects were also assessed using Menidia beryllina, aeuryhaline species. Effects of endocrine disruptors like 17β-ethinylestradiol (EE2) were monitored in three generations. Malformations were seen also in non-exposed offspring. Epigenetic analysis will be performed to assess transgenerational inheritance via epigenetic mechanisms, a topic which is also emerging in the field of toxicological fish research. Rainbow trout (Oncorhynchus mykiss) were applied as a model to gain further insights into polycylclic aromatic hydrocarbon (PAH)-induced cardiotoxicity. Different PAH’s induced various changes at the level of the transcriptome, and for some genes, direct effects on ion channels were shown. The species Percilia irwini were used by another study to investigate water quality in a river in central Chile. Percilia irwini were collected along the river and a variety of biomarkers (weight, size, EROD activity, gene response) were assessed and an Integrated Physiological Biomarkers Response (IPBR) was calculated. The session was closed with two poster spotlights. One was trying to make a link between gene expression patterns and in vivo endpoints like movement, heart beat and malformations. The second one presented new insights on cross-species differences in the modulation of human and zebrafish nuclear receptors by single chemicals and environmental mixtures.
Following the oral presentations a poster session was held with nearly 50 posters about a variety of different toxicological studies using different fish models. Different toxicological mechanisms like endocrine disruption, neurotoxicity and carcerogenicity were presented, including studies focused on specific compound groups (i.e., PAH and metals).
The poster session was closed by a poster corner focusing on studies using complex environmental samples. One study used caging experiments as a tool for in situ assessment of neurotoxic effects. Another study focused on effects of treated sugar cane vinasses on gills of Nile tilapia (Oreochromis niloticus). Violet Goby (Gobioides broussonnetii – Gobiidae) were used to assess toxic effects from one of the most productive estuaries in Brazil. Caging experiments with rainbow trout were used to assess the effects of ozonation on waste water treatment quality. The RADAR (Rapid Androgen Disruption Adverse Outcome Reporter) assay was presented as a novel medium-throughput tool to assess androgen disruption in environmental samples. The final poster in the corner discussed the results of environmental samples collected near vineyard parcels on rainbow trout larvae (Oncorhynchus mykiss).
Conclusion From the Session
The session clearly showed that a big step forward has been made when applying fish model species for toxicological studies. State of the art methods are now usable for many different species due to the ever-increasing amount of genome assemblies and more accurate annotations of genes. RNA sequencing and liquid chromatography-mass spectrometry proteomics are widely applied. Thereby, visual malformations can now be linked to molecular mechanisms. Better understanding of compound metabolism and uptake in fish further improves understanding of toxicological processes. More studies are now focusing on transgenerational and epigenetic effects, which is clearly aided by the fast development in fish model research and established methodologies for epigenetic research. As a novel endpoint, which receives much attention in the field of toxicology, were the effects on the microbiome. The times of simple dose–response lethality curves, identifying only at what concentrations toxicity is caused, have clearly passed. Fish models now help to understand how compounds cause toxicity. Despite this progress, the presented data was barely used for risk assessments. We hope that further studies will help to make findings in fish models more accepted in toxicological risk assessment.
Based on the great success of this session, we are looking forward to the contributions for the SETAC Europe 29th Annual Meeting, which will be held from 26–30 May 2019 in Helsinki, Finland.
Laurence Deydier Stephan, European Chemicals Agency (ECHA); Sonia Manzo, Italian National Agency for New Technologies Portici Research Centre (ENEA); Simona Scalbi, Bologna Research Centre (ENEA); and Doris Voelker, German Environment Agency (UBA)
In the EU, nanomaterials are implicitly covered by the substance definition of the REACH (Registration, Evaluation, Authorisation and Restriction of Chemicals) Regulation, even if nanomaterial-specific obligations will only enter into force in January 2020. Other regulations, such as the Biocidal Products Regulation (BPR) and the Cosmetics Regulation, already consider them explicitly.
As a regulatory agency, the European Chemicals Agency (ECHA) is proactive in the international debate on the regulation of nanomaterials. Together with other stakeholders, such as competent authorities and scientific institutions, the agency works to have the most recent scientific research developments integrated into the relevant regulations. Regulators have recognized the need for new or updated standardized test methods, guidance and approaches. Accordingly, efforts have been made to strengthen the implementation of existing methods and guidelines and develop new ones. Research activities have been initiated worldwide to promote more appropriate and representative nanomaterial risk assessment. Furthermore, the scientific and regulatory communities have progressed in the development of life cycle-based approaches for eco-design of new nano-enabled products as well as grouping approaches specific to nanomaterials.
The session covered the major findings and latest developments of regulatory relevance in the risk assessment of nanomaterials.
The session consisted of six platform presentations and 14 posters. Two presentations focused on the regulatory situation under REACH, BPR, CLP (Classification, Labeling and Packaging Regulation) and the Canadian Environmental Protection Act (CEPA), while one provided an overview on reliable tools and methods currently available for risk management and safe innovation in Europe. Three presentations covered scientific advances on nanomaterial behavior and effects. The safer-by-design concepts and advanced approaches for refined environmental risk assessment through test strategies developed under the 7th Framework Programme for Research and Technological Development (FP7) Horizon 2020 projects such as NanoREg2, EC4Safe Nano, and NanoFase were also presented and discussed. The platform presentations sparked discussion and interest among the 50 participants that continued through the poster session.
Anu Kapanen of ECHA provided an overview of the agency’s regulatory and scientific activities, with a particular focus on the work related to nanomaterials and the tools used to assess their hazards and risks. As a science-based regulatory body implementing the EU chemicals legislation, the agency complements the formal, legal instruments available under the different regulations with softer approaches to support and advise stakeholders, including expert groups, as well as various communication and exchange platforms, such as the European Union Observatory for Nanomaterials (EUON). The EUON website and its structure drew several questions from participants after the presentation; other topics discussed included the European Commission’s recent decision to adopt specific requirements for nanomaterials in the REACH annexes and ECHA’s upcoming update for guidance on information requirements and chemical safety assessment specific to nanomaterials.
Marie Claude Sauvé, Environment and Climate Change Canada, presented the development of a risk assessment framework under CEPA for nanomaterials. The framework, based on identified scientific challenges in nanomaterials testing and data gaps for prioritization, aims to improve environmental risk assessment of nanomaterials. While Europe has regulations allowing direct requests for information and testing, under CEPA, Canada needs to first collect information from existing literature, international programs or open databases before assessing what potentially could be the need or risks of collecting and requesting further information. A web page will be launched soon to initiate and motivate collaboration and discussion on critical data and knowledge gaps among regulators, scientists and industry.
The first two presentations were followed by a question and discussion period on the use of data and the potential to strengthen collaboration within and beyond Europe so that, among other benefits, the work carried out by Canada and by ECHA could feed into each other.
Simona Scalbi, Bologna Research Centre – ENEA, presented the preliminary findings of the EC4SafeNano project, set up to develop a distributed center of European organizations offering services for risk management and safe innovation for nanomaterials and nanotechnologies. One of the first actions of the project is the development of a validated inventory of tools, trainings and best practices available in human and environmental nanosafety, available via a website. The inventory also includes quality criteria, such as the level of evaluation and validation of the resource and its acceptance under REACH. At this stage, the findings show that training is the most markedly missing element.
Andrew Barrick, Bretagne Loire University, from the NanoReg2 project, presented some recent developments related to safer-by-design strategies for nanomaterials and environmental safety. Studies applying Organization for Economic Co-operation and Development (OECD) test guidelines and in vitro or in vivo testing strategies have been carried out to assess toxicity of different types of carbon nanofiber. The results obtained using characterization methods, standard test guidelines using algae and daphnids, and in vitro or in vivo testing of mussel haemocytes have shown carbon nanofibers to exhibit different levels of toxicity at different concentrations and durations of exposure dependent on the manufacturing process. Interestingly, differences in in vitro toxicity of carbon nanofibers were dependent on the dispersion used for the environmental test media (with and without bovine serum albumin), indicating that the method used to prepare suspensions can affect toxicity, with obvious implications for comparing interspecies toxicity results. While this research promotes safer production design of carbon nanofibers, this does not necessarily ensure safer use of products.
Jerome Labille, French National Centre for Scientific Research, CEREGE Laboratory, presented research on sunscreen formulations that use a different functionalization of titanium dioxide (TiO2), grounded on an eco-design approach that considers all lifecycle stages in the risk assessment, from fabrication through to the end-of-life of the product. This research, carried out with an industry consortium, seeks to minimize the risks posed to consumers and the environment by lowering either the amount of TiO2 released or the hazard of the product. Different compositions have been tested. The first results on dispersion and size distribution in seawater media show that silica-coated TiO2 loses its coating once placed in water for aging, leaving bare and photocatalytic TiO2 in the environment. Further work related to beach and seawater monitoring and the testing of potential skin irritation are planned to determine which formulation delivers the best compromise between efficiency as sunscreen and the hazards connected to exposure levels.
Bernd Nowack, Swiss Federal Laboratories for Materials Science and Technology, illustrated some research on modeling nano-specific environmental risk assessment in different regions of Europe. For this, he presented the environmental risks of five different nanomaterials based on a dynamic probabilistic model flow analysis to predict their environmental concentrations. These data were further compared to effect concentrations assumed to be of no hazard to environmental organisms, derived from probabilistic species sensitivity distributions using ecotoxicological data for each nanomaterial. First results show that while some nanomaterials in specific regions will pose a risk due to their potential fate, release and known hazards, others may be of no concern. All these estimations include uncertainties and will be more reliable if and when proper data on production and recycling is available to researchers. This work will be continued and further developed for local scale modeling and the recycling stage, which is one of the known sinks and potential local hot spots for release.
Conclusions From the Session
The session made clear that progress has been made in nanomaterials safety, by way of chemicals regulation, improvements in environmental risk assessment and safer-by-design concepts. Current emphasis lies on the systematic investigation of the status quo regarding the knowledge on human and environmental nanosafety, the implementation of adequate standards and tools for risk assessment, as well as on the communication of these findings. Enhanced interest and effort in comparing the fate and effects of different nanoforms will support relevant environmental risk assessment approaches (e.g., in terms of grouping forms or even materials) as well as safer innovation of nanomaterials by applying safer-by-design tools as a pre-regulatory step.
Questions and issues raised by the audience demonstrated that despite headway made in the regulation and environmental risk assessment of nanomaterials over their lifecycles, there is a still a need for much discussion. The legal obligations may provide opportunities not only to generate data but also to promote the gathering and sharing of all the available research findings and tools, which can in turn assist in deciding on further testing strategies that will promote research innovation towards safer nanomaterials.
Laura Cutaia and Grazia Barberio, ENEA, and Serena Righi and Alessandra Bonoli, University of Bologna
Emerging technologies are often considered a benefit for future generations and a key for a better life quality and a safer environment. They are helping to solve some of the most challenging issues of the twenty-first century, including problems regarding health, food, natural resources and the environment. However, emerging technologies can be highly reliant on mineral resources; therefore, there is an increasing concern on what risks for the development of future technologies and the supply of raw materials (RMs) could entail or which environmental and social impacts these technologies might impact.
The aim of this session was to investigate how the adoption of emerging technologies can affect the consumption of RMs. The way proposed was the combination of life cycle thinking (life cycle inventories, specific datasets, etc.) and RMs requirement and management scenarios (RMs requirement analysis over the life cycle chains, market conditions and trends, etc.).
The session was well received by the SETAC conference members, with approximately 100 participants.
The session was opened by the chair Laura Cutaia, ENEA, who pointed out that emerging technologies are becoming increasingly important in the very process of innovation, but it is essential to pay attention to uncertain side effects of their implementation, such as future end-of-life problems and the use of critical materials. The session featured five oral presentation and three poster spotlights.
The first speaker was Silvia Maltese, University of Bologna, who reported a case study related to the automotive sector. The study concerned the substitution of traditional glass fibers with an innovative bio-composite material (with woodchip content). Discussion on impact for wood production, other use of wood, source of date and economic assessment was stimulated following this case study.
Maria Anna Cusenza, University of Palermo, presented a life cycle assessment of a lithium-ion battery cell focused on resource depletion. The LMO-NMC battery cell, which is Li-manganese and nickel-manganese-cobalt, was evaluated as it can present a good compromise between the higher and the lower performance of the NMC and LMO technologies, respectively. Together it can guarantee lower cost and lower vulnerability to supply disruption due to the lower cobalt content. Details of future use and impact categories were provided in follow up questions from this presentation.
Giovanni Mondello, University of Roma Tre, also considered the transport sector. The study evaluated the environmental burdens of a stand-alone charging station for electric bicycles. The analyzed station was composed of eight positions for charging the e-bicycles’ batteries and of two wind-solar modular systems for energy production. Discussion on the avoidance of impact and the substitution of other type of energetic sources were part of this presentation.
The next speaker was Cristian Chiavetta, ENEA, who investigated the requirements of raw materials in future scenarios of electric technology and the penetration in the urban vehicle fleet. The study went beyond the quantification and estimated raw materials availability from a market perspective and what is defined as the limiting factor among three basic materials of the current electric technologies (lithium, graphite and manganese). Details on lifespan choice and sensitivity analysis of different lifespan time were provided in this presentation.
Ariane Albers, IFPEN, presented a hybrid approach for assessing the French energy transportation over long timeframes (2007 to 2050). The approach combines the MIRET energy systems model with dynamic Cbio accounting models towards dynamic life cycle assessment (LCA). The former is a prospective techno-economic partial-equilibrium model; the latter assesses biomass growth and allometric relations representing the carbon fixation of a vegetation species. Discussion following this presentation was stimulated on relevance of time horizon choice for providing results to policy makers.
The poster spotlight presentations were given by Esmeralda Neri, University of Bologna; Fabio Grimaldi, University College London; and Natalya Tsoy, Leiden University. Neri described a system that allowed the quantitative recovery of carbon fibers contained in the initial reinforced composites and avoided the consumption of material and energy deriving from the production of virgin carbon fibers. Grimaldi analyzed a wide selection of engineered nanoparticles production, notably silver, gold, silicone oxide and iron oxide comparing conventional batch production and the innovative continuous micro/milli-flow synthesis. Tsoy’s poster consisted of an anticipatory life cycle assessment of a novel anti-reflective coating for greenhouses, which are expected to have higher light transmittance than the conventional coatings by 2.5%. The other posters presented during the session included studies involving the chemical and pharmaceutical sectors, nanoparticles production, waste water treatment as well as the energy sector.
The following conclusions can be drawn from all of the platform and poster presentations:
Emerging technologies can impact the RMs market and can influence the their availability in terms of valorization of “urban stock.” Even though the session covered a large spectrum of sectors and demonstrated that emerging technologies have an essential role in many areas, the number of the studies concerning transport and energy highlighted that these sectors are particularly dynamic and interested in the role of innovation within these sectors. The results of the presented research also show that in most cases, the innovative technologies provided environmental benefits with respect to the traditional technologies that they might replace, and LCA can be used as a tool for quantification of dynamic changes, with a particular focus on consequential LCA or anticipatory LCA. Moreover, the studies demonstrated through the information provided, they can support strategies, policies, plans and programs on resource efficiency, resource management and inform on the circular economy.
Summaries Published in the July 2018 Issue
Xindi Hu, Harvard University; Rainer Lohmann, University of Rhode Island; and Jon Benskin, Stockholm University
Past and ongoing production and use of legacy and alternative poly- and perfluoroalkyl substances (PFASs) has been recognized as one of the biggest challenges in chemical management. With the development of advanced chemical analytical methods, hundreds of highly fluorinated compounds have been detected in humans and the environment. However, considerable gaps exist in linking the vast amount of data to actionable strategies for reducing wildlife and human exposures and associated adverse health effects. This is due to the limited understanding of physical–chemical properties of alternative PFASs, relative importance of environmental and human exposure routes, and variable mechanisms of toxicity across compounds and across species. This session provided a forum to share advances in monitoring and modeling efforts that help to better quantify exposure to PFASs and associated health impacts.
Strong interest for the session was expressed through 28 high-quality presentations and more than 70 participants. Principal investigators, postdoctoral scientists and three graduate students gave six platform presentations, which were organized in the order of advancements in analytical chemistry, evaluation of physical chemical properties, environmental fate and exposure modeling, and remediation.
Gabriel Munoz from the University of Montreal, Canada, kicked off the session with a talk on development of a method for measuring a wide range of PFASs in soil samples at aqueous film-forming foam (AFFF)-impacted sites. Current methods for analysis of perfluoroalkyl acid (PFAA) could seriously underperform for certain newly identified PFASs. His talk proposed a suitable preparation procedure for the multi-residue analysis of PFASs that improved the method recovery for many PFASs, such as betaines (improved from 30–50 % to 70–105%). Ongoing work involves method development for PFASs in biota to support better evaluation of PFAS bioaccumulation. Munoz presented a companion poster titled “Assessment of persulfate oxidation liquid chromatography tandem mass spectrometry for the analysis of perfluoroalkyl and polyfluoroalkyl substances in water.”
Raphael Janousek from the University of Applied Sciences, Germany, presented his work on PFASs in products used in the building industry and industrial textiles. A total of 23 samples from products used in the building industry and 28 industrial textiles were investigated. Monitoring covered 29 PFASs with chain lengths from C4 to C14, including carboxylic acids, sulfonic acids, sulfonamides and fluorotelomer alcohols (FTOHs). FTOHs accounted for more than 80% of the sum of PFASs detected. In addition, he a worst-case scenario calculation was presented to estimate the emission potential from the entire building industry in Germany.
Clifton Dassuncao from Harvard University, USA, presented findings from a longitudinal cohort study among a seafood-consuming population in Faroe Island. A variety of novel tools were used, including advanced statistical analysis and toxicokinetic modeling. Despite the global efforts to reduce production and emission for long-chain legacy PFASs, work done by Dassuncao and colleagues suggested PFAS concentrations in seafood have not decreased as rapidly due to their extreme persistence in the marine environment. Over the study period of 1993 to 2012, PFAS exposures from consumer products have become increasingly important.
Steven Droge from University of Amsterdam, The Netherlands, presented his work that filled an important data gap in understanding the physical–chemical properties of a wide variety of PFASs. A model based on quantum chemistry, COSMOtherm, was able to predict both the membrane-water partitioning (Kmw) and pKa of emerging ionic PFASs. However, experimental data using solid supported lipid membranes highlighted that COSMOtherm misinterpreted the membrane affinity of anionic perfluorinated surfactants. This work has important implications for environmental fate modeling and risk assessment of PFASs.
Charlotte Wagner from Harvard University, USA, presented a large-scale modeling exercise on the burden of perfluorooctane sulfonate (PFOS) in the global oceans. Using a global marine circulation model, MITgcm, and an updated spatially resolved emission inventory from Europe, North America and China, this work estimated that historical total discharge into the ocean from Europe was to a similar amount as that from North America. The contribution of Chinese emissions to the PFOS concentration in the surface ocean currently is small but may increase over time.
The session ended on a positive note with a talk focusing on solutions, given by Gijs Breedveld from the Norwegian Geotechnical Institute. He presented two case studies on identification of point and diffusive source of PFAS inputs, environmental behavior in multiple environmental media as well as suitable remediation methods for different sites. The first case study was at Oslo airport, where AFFF was used for firefighting purposes. Two remediation methods using sorbent amendment and in situ soil washing showed promises to reduce leaching and improve ground water quality. The second case study was in Tyrifjorden, where PFAS profiles were identified in different sources to determine their distinct chemical signatures.
The talks and posters presented at this session highlighted the scientific challenges faced by society in managing the entire class of PFASs. Due to their unique surfactant properties and deviation from traditional persistent organic pollutants, innovative methods are needed to effectively assess and manage PFASs. By integrating up-to-date knowledge, highlighting state-of-the-art research tools and identifying critical gaps for decision-making, this session aimed at advancing efforts to reduce ubiquitous exposure to PFASs, avoiding regrettable substitution and reducing risks to public health.
Werner Brack and Rolf Altenburger, Helmholtz Centre for Environmental Research – UFZ; Jos van Gils, Deltares; and John Munthe, IVL Svenska Miljöinstitutet
While the Water Framework Directive defines a holistic ecological status to monitor and assess the quality of aquatic ecosystems, chemical status is defined in a very traditional way focusing on 45 so-called priority substances. This approach ignores non-regulated and unknown (emerging) pollutants as well as the effects of mixtures. A recent study in the process towards a non-toxic environment led by the European Commission highlights several environmental management issues and recommendations including:
- Improved identification and tracking of all substances meeting the criteria for substances of very high concern (SVHCs) and including very persistent substances as well as substances of concern meeting other endpoints not yet adequately addressed (e.g., endocrine disrupters, neurotoxins, immunotoxins and developmental toxins)
- Additional hazard identification and risk assessment processes that allow for more rapid screening and identification of potential chemicals of concern and that can cope more efficiently with the large numbers of existing chemicals as well as the ever increasing numbers of new chemicals being invented and placed on the market
Scientific and technological progress in effect-based and multi- and non-target chemical screening opens new horizons for holistic monitoring as demonstrated for example in the large EU project SOLUTIONS. Moreover, high-throughput integrated modeling is becoming capable to predict emissions, fate and transport, and exposure from and risks to aquatic ecosystems and human health for thousands of chemicals to rivers in Europe. Increasing knowledge about the efficiency of abatement options and packages, through the use of integrated modeling, helps to find tailor-made solutions to minimize chemical footprints and estimate toxic risks. This special session demonstrated how the integration and mutual validation of such approaches could open new opportunities to achieve a non-toxic environment. The session brought together experts in effect-based monitoring, analytical chemistry, mixture assessment, exposure and effect modeling, abatement measures and science-policy interfacing from academia, European and national regulation, industry and non-governmental organizations to maximize the impact of these new ideas.
The session included 18 presentations starting with the perspective of non-governmental organizations, industry and regulation represented by Manfred Santen, Greenpeace; Milou Dingemans, KWR; and Manuela Helmecke, German Environment Agency, UBA. Santen highlighted that wastewater treatment is not able to deal effectively with hazardous substances so that the problem also needs to be tackled at the source, for example the elimination of perfluorinated compounds from textiles manufacturing, and the needs to involve large international retail brands. He challenged scientists to identify hazardous chemicals in a timely manner. Dingemans stressed the need for and the promise of effect-based monitoring and the necessary trigger values to safeguard water resources. According to Helmecke, the review and possible revision of the Water Framework Directive provides a suitable window of opportunity for implementation of such new approaches. The feasibility of a proposed revision for all member states is a pre-requisite, and therefore UBA intends to evaluate several new monitoring tools and methods during the upcoming 4th Joint Danube Survey.
In the subsequent block, these general perspectives were supported by presentations on novel monitoring tools that have been successfully demonstrated in comprehensive case studies. Emma Schymanski, University of Luxemborg, fascinated the audience with the combination of non-target screening for “real-time” monitoring as well as retrospectively via “digital freezing” platforms. Her contribution highlighted the strength of open science and world-wide (big) data sharing practices. This chemical analytical approach was complemented by Beate Escher, Helmholtz Centre for Environmental Research – UFZ, highlighting the capabilities of toxicological profiling with cell-based and low-complexity whole organism assays. Escher completed her presentation with a generic approach to define effect-based trigger values, which serve a goal similar to the well-known concentration-based quality standards. She distinguished between specific endpoints where a large share of the observed effect can often be explained from known chemicals and apical endpoints where effects may be caused by the complex mixture of many compounds. Anthony Schroeder, University of Minnesota, highlighted chemical–gene interactions networks to develop knowledge assembly models, again building on shared data from existing sources. Using the mapping of transcriptome data from fathead minnows exposed to wastewater treatment plant effluents, he could evaluate the likelihood of a chemical to contribute to the detected effects. Werner Brack, Helmholtz Centre for Environmental Research – UFZ, demonstrated integrated chemical and biological tools for the identification of drivers of effects detected by effect-based monitoring. Using the example of the fluorescent dye coumarin 47, which was found as an endocrine disruptor in the environment for the first time, he demonstrated that this approach often unravels unexpected toxicity drivers that remain ignored in chemical monitoring. He also showed to the packed meeting room how fractionation and mixture toxicity experiments can reveal synergistic effects in complex environmental mixtures.
Since contamination with chemical mixtures is the normal case rather than an exception, the session put a strong emphasis on risk assessment of mixtures. Roman Ashauer, University of York, pointed out that aquatic organisms are not only exposed to mixtures at the same time but also in sequence and stressed that not only the dose makes the poison but also the sequence. Rolf Altenburger, Helmholtz Centre for Environmental Research – UFZ, presented the conceptual framework used in the SOLUTIONS project to deal with mixtures of pollutants in water resources management, coming back to the specific uses of apical assays for monitoring purposes and specific assays for diagnosis. Andreas Kortenkamp, Brunel University, explained the human-health–related mixture risk assessment for pollutants via the uptake of fish contaminated by polychlorinated dioxins and polybrominated diphenyl ethers. Based on data from the Joint 3rd Danube Survey, he showed that according to state-of-the-art human risk assessment practices the analyzed fish should not be eaten. Michael Faust, F+B, then moved back to water pollution and addressed the prioritization of pollutants and mixtures based on different lines of evidence including ecological, effect-based and chemical monitoring as well as modelling. Faust argued that his recommendations would not be able to find their way into standing Water Framework Directive legislation unless certain aspects would be addressed in the upcoming revision. A convincing application of Faust’s approach was presented by Thomas Backhaus, University of Gothenburg. He described the impact of complex mixtures from wastewater treatment plant effluents and the recovery of the biofilm community after the upgrade of the wastewater treatment plant.
Monitoring-based approaches can be strongly supported by high-throughput exposure and risk modelling. This was demonstrated by Jos van Gils, Deltares, using the SOLUTIONS “model train” for the spatially explicit prediction of the concentrations of about 1,800 chemicals validated in the rivers Danube and Rhine as well as several Spanish and Swedish rivers. He subsequently presented work by Andreas Focks, Alterra, who used Individual Based Models to translate predicted concentrations into simulated inhibition of population growth rates and hence the chemical impact on population viability on the European scale. He also demonstrated the application of widely used msPAF modelling, on a larger spatial scale and for more chemicals than ever before. The meaning of such results was discussed by Leo Posthuma, RIVM, using eco-epidemiology as a strong tool to liaise ecological status to mixture toxic pressure, based on large sets of surveillance monitoring data. This is also an important step towards unravelling mixtures in the context of multiple stress, as presented by Sebastian Birk, University of Duisburg-Essen, who highlighted the conclusions of the EU project MARS and the implications of toxic stress as assessed in the SOLUTIONS project.
In a final block of presentations, Annemarie van Wezel, KWR Watercycle Research Institute, and John Munthe, IVL Svenska Miljöinstitutet, closed the loop back to the stakeholder perspectives from the beginning of the session, addressing mitigation options for emerging chemicals and future perspectives. She presented the current knowledge of removal efficiencies of advanced water treatment technologies, while also highlighting the power of measures earlier in the chemicals’ life cycle. Munthe briefly discussed the different existing legislative frameworks and emphasized the need for dialogue, transparency and exchange of knowledge, and co-presenter Dirk Bunke, Öko-Institut, explained the implications of developments in public health, food production, urbanization and technologies for the identification of future pollution challenges.
Complex mixtures give distinction to water quality rather than individual (priority) pollutants. Powerful monitoring approaches are available to detect contamination and toxic stress and the associated links to both. Modelling may strongly support these approaches and help to evaluate best abatement options. A solutions-focused approach rather than status assessment alone is of key importance for approaching the goal of a non-toxic environment. This requires transparency and openness of information as well as increased international cooperation in science and regulation. Integrated European projects on applied research themes with strong stakeholder involvement such as MARS and SOLUTIONS are milestones in this process, and their results embody a strong plea for European research.
Sofia Augusto and Nuno Ratola, University of Porto, and Mira Aničić Urošević, Institute of Physics Belgrade
This session aimed at giving an overview of the current biomonitoring tools and strategies used towards the protection of the environment. Besides unintentionally produced contaminants, hundreds of high production volume chemicals on the market cannot be properly assessed for risk without bioaccumulation data in registered dossiers. Certain ecological groups, sensitive to contaminants, can be used as sentinels of emerging contaminant problems.
The session hosted contributions regarding environmental monitoring of inorganic and organic contaminants using terrestrial ecological biomonitors (plants and animals) and covered a wide range of contaminants, being specifically focused on bioaccumulative, toxic and persistent compounds.
The key points for the six presentations included:
- The session started with Annekatrin Dreyer, Eurofins, who presented results of the evaluation of 123 selected persistent organic pollutants (POPs) in mosses within the 2015 European moss survey. The findings were further compared with results derived for deciduous tree leaves and coniferous shoots (needles) collected from the German Environment Specimen Bank (ESB).
- Stefan Nickel, University of Vechta, provided the audience with an example of how mosses can be used to monitor heavy metal and nitrogen concentrations at a national scale over time. By mapping percentile statistics of element concentrations in moss collected from 1990–2015 in forests throughout Germany, the authors could observe temporal and spatial trends for some priority pollutants according to the Convention on Long-range Transboundary Air Pollution (CLRTAP).
- Fabrizio Monaci, University of Siena, presented the results of a biomonitoring study using holm oak leaves and pine needles from Southern Tuscany, known for its long mining history. The author focused on sources of variability (e.g., different age classes) when using these plants to monitor heavy metals and nutrient concentrations and how these can be overcome for a better interpretation of long-term environmental monitoring results.
- The use of bat guano to assess the historical exposure to inorganic contaminants was introduced by Lauren Gallant, University of Ottawa. Gallant and her colleagues, examining two bat guano deposits from Jamaica dated over 3,000-years-old, were able to reconstruct the agricultural and industrial revolution history of the country, the introduction of leaded gasoline as well as the peak of nuclear weapon testing.
- Velesia Lesch, North-West University, explored for the first time the use of dragonflies as potential biomonitors of environmental metallic elements and perfluoralkyl substances (PFAS). Results indicated that farming areas had significant lower PFAS concentrations than industrial areas and that the presence of mining and wastewater treatment plants showed an impact in terms of metal contamination.
- In the last presentation of the session, Lakmini Egodawatta, University of Wollongong, focused on the impacts the ageing of agricultural lands has on the co-contamination of arsenic and antimony on edible plants. The author concluded that antimony contamination in agricultural soils poses a greater human health risk and hazard when in presence of arsenic, as antimony accumulates in edible crops with no observed phytotoxicity or reduction in vegetable productivity.
The successful session featured six platforms from four different continents, balanced between young researchers and more experienced scientists, and provided a wide spectrum of uses for biomonitoring strategies. With an excellent turnout, the session had a strong audience participation, fostering further discussion among the participants.
Biomonitoring was again recognized as a very important tool not only to understand the dynamics and response of the ecosystems, but also as an indirect way to potentiate wide monitoring and legislation enforcement regarding priority contaminants.
Lastly, the need to involve the general population on ecological biomonitoring studies (as direct participants or through informational meetings) was considered essential to raise awareness of both the tools and the problems to tackle.
Gudrun Obersteiner, BOKU University of Natural Resources and Life Sciences, and Michele De Rosa, 2.-0 LCA Consultants
Adequate interpretation of life cycle assessment (LCA) results is important to ensure that the related information is correctly used to inform interested stakeholders and decision-makers. However, LCA remains in all cases a modeled system that only reflects key elements of the real life system. A description and assessment of this model system requires simplifications, streamlining, assumptions, filling data gaps, cross reading from other sources of information or the application of further models (e.g., for impact assessment), just to name a few. Decisions made in relation to the assessment of such modeled systems will in turn affect the results, their representativeness, scope of validity and ability to answer the research questions set out in the goal and scope of a study.
Although this topic has been in discussion since the early 1990s, uncertainty seems to be one of the most unsolved problems within LCA. Only when we know how reliable the outcomes of LCA results are, then we can use them in an effective way as part of decision-making. This requires information as to what extent the outcome of an LCA is affected by various types of uncertainty such as parameter, scenario and model uncertainty. Currently, a best practice is still missing, and uncertainties are often not considered or addressed in LCA result presentation. These limitations might even lead to wrong decisions.
Therefore, this specific session was held to address this topic. A high interest for the session was expressed through a large attendance of approximately 200 people. This session not only dealt with the final interpretation representing the last stage of an LCA, but it also included the other stages of LCA studies where uncertainties can occur. Currently the majority of LCA studies do not include data uncertainty and uncertainty analysis. Due to the high variability, LCA results should be interpreted as a distribution of values rather than a single “true value.”
Presentations during this session included:
- Reinout Heijungs, Vrije University, summarized recently published work on how to treat uncertainty in LCA. Previous works did not account for correlation. Heijungs described two types of correlations: correlated samplings and correlated results. Dependent sampling implies that process data for the product alternatives being compared are sampled based upon one and the same random drawing of parameter values, resulting in identical data sets for this shared process. As an example for correlated results, Heijungs used transport processes as the process input of diesel is always related to the process output of CO2 (emission); if the process consumes more diesel for the same amount of transport, the CO2 emission will also increase. An analysis of the uncertainty propagation and correlation should, therefore, always be provided.
- Mélanie Douziech, Radbound University, presented on the variability and uncertainty using personal care products as a case study, which was based on the chemical footprint. The calculated chemical footprints spanned nearly four orders of magnitude. This wide span could be attributable to fragrances (61%), surfactants (20%) and the amount of product used (16%). The significant contribution from fragrances and surfactants can largely be explained by the uncertainty in their environmental impacts.
- A combined uncertainty and scenario analysis within LCA of waste management systems was introduced by Valentina Bisinella, Technical University of Denmark – DTU Environment. Bisinella presented a novel method for combining uncertainty and scenario analysis, illustrated by a case study with three hypothetical waste management options for treatment of residual household waste in the municipality of Copenhagen extending through 2025.
- Antoine Esnouf, French National Institute for Agricultural Research (INRA) Laboratory of Environmental Biotechnology, focussed on the relevance of specific impact categories for LCA results interpretation to avoid uncertain conclusions because of challenges caused by the wide range of environmental impacts results. Esnouf proposed a novel methodology as to how practitioners could benefit by selecting impact categories to focus the interpretation of the results with regards to relevant environmental issues.
- One major reason for uncertainty is spatial variability. Regionalization of life cycle inventories can therefore help increase the geographic representativeness of the model, but this is connected with huge efforts for data collection. Laure Patouillard, CIRAIG, proposed in his presentation of a procedure to prioritize regionalization efforts based on global sensitivity analysis (GSA) to reduce the spatial uncertainty of LCA.
All of the presenters gave insightful views as to the range of uncertainty topics within LCA modelling, starting with data quality, providing solutions concerning modelling assumptions but also focusing on characterization factors and impact category selection. Once more the importance of including uncertainty assessment for reliable LCA results was pointed out in these presentations. One should always to be aware that results of an LCA are uncertain due to lack of knowledge about the true value of its model parameters. Therefore, presenting one single value as output of an LCA could be misleading, and in the worst case, may lead to wrong decisions.
Gerardo Pulido-Reyes, Autonomous University of Madrid, and Roberto Rosal, University of Alcala
Nanomaterials have turned into ecological and public health problems of great importance due to their extensive uses and broad applications. During the last few years, considerable research has been performed related to nanomaterials and their possible biological impact on the biota and human health. However, the knowledge of their distribution, transformations and abundance is far from complete and requires further evaluation. The knowledge of the accumulation potential, sources, sinks and real environmental impacts of nanomaterials is also currently limited. The published studies to date are only focused on pristine or manufactured nanomaterials where modifications of the materials or experiments mimicking real scenarios have not been fully assessed. Another research topic currently receiving a lot of attention is related to plastic pollution. Plastic particles (micro- and nanoplastics and nanopolymers) represent environmental contaminants of high importance due to their low degradation rate and their high persistence in the environment. The growing amount of plastics which are being used in many industrial fields derived for their excellent material properties, such as low weight, easy processing and high stability against various chemical degradation, may also increase their undesirable presence in the environment. It is worth noting that much of the recent concern has been focusing only on microplastics, but almost no data has reported the impact of nanoplastics or nanopolymers.
This session was focused on experimental studies that highlight the importance of assessing the distribution and, also, the biological effects of nanomaterials and plastics under more relevant environmental conditions. After reviewing all the high-quality contributions received, six platform presentations and twenty-six posters were delivered in the session.
There is a lack of knowledge regarding the abundance of microplastics in the environment. The first presentation by Mark Hartl, Heriot-Watt University, addressed this problem and aimed to contribute to the development of a previously lacking, quantitative long-term marine microplastic database. The results of a three-year pilot project in the Firth of Forth, United Kingdom, was presented. It was shown there are high numbers of plastic particles and fibers along both shores of the estuary. The microplastic concentration remained stable along the sampling time, and no apparent pattern of spatial distribution of neither particles nor fibers was observed. This study has established a significant baseline set of information and will help future research better track the contamination by microplastics.
The second platform presentation focused on understanding the effect induced by nanoparticles (gold and polystyrene nanoparticles) towards a model freshwater microalga and discriminate the responses of the microalga in comparison with known stress mechanisms, such as nutriment deficiency, light deficiency and metallic stress, using infrared spectroscopy. Maureen Déniel, University of Le Mans, showed that the different stresses involved chemical modification of the microalgae leading to variations in the infrared spectra and that this technique, coupled to a multivariate analysis promises to be a useful tool to rapidly discriminate different stress effects on the model microalgae Chlamydomonas reinhardtii.
During the third presentation, Gerardo Pulido-Reyes, Autonomous University of Madrid, showed the biological effects of two nanopolymers, highgeneration cationic poly(amidoamine) dendrimers of generation 5 and 7. After using a wide panel of techniques, it was clearly demonstrated that these polymers can induce a severe toxicity to Anabaena sp. PCC7120 and provoke several physiological, morphological, photosynthetic and genetic alterations. This study provided relevant information for the environmental risk assessment of this nanomaterial.
Due to their multiple applications, there is a great concern over the potential impact of nanomaterials on environmental health, either alone or in combination with other anthropogenic contaminants. The presentation given by Awadhesh Jha, University of Plymouth, focused on testing the hypothesis that environmentally relevant carbon-based nanoparticles, C60 fullerenes and multi-walled carbon nanotubes, and polycyclic aromatic hydrocarbons as benzo(a)pyrene can interact with each other to differentially modify their potential toxicity. The biological response of marine mussels showed variable sensitivity with respect to exposures and co-exposures with the different nanomaterials and the organic compound. This study underlined the complexity of the environment, regarding the potential effects of nanomaterials along with other environmental toxicants.
The last two platform presentations assessed the effects of copper oxide nanoparticles (CuONPs) under more relevant environmental conditions. Tobias Lammel, University of Gothenburg, addressed the transfer of CuONPs and dissolved copper, administered as CuCl2, from sediment to worms (Tubifextubifex) and further from worms to fish (Gasterosteus aculeatus). This bioaccumulation study concluded that the transfer of CuONPs along the food chain may be limited compared to dissolved copper. Moreover, the work presented by Laure Giamberini, Université de Lorraine, was focused on the fate and effects of different CuONPs on a widespread endobenthic freshwater bivalve within a mesocosm study. It was shown that CuONPs affected some biological biomarkers and also the bivalve behavior by increasing burrowing, suggesting an avoidance reaction. Both works dealt with setting up a more complex design of exposure, which may help gain more environmental realism.
All talks were well received and the attendees, more than a hundred people for most presentations, engaged in a lively discussion. After this stimulating session, it is necessary to highlight the importance of addressing eco(nano)toxicity from a more realistic point of view and to develop robust methods and techniques to help monitor the presence of anthropogenic particles, nanomaterials and micro- and nanoplastics in the environment. All this information will be useful to reduce the uncertainties which surround nanotechnology and to shed light regarding the current problem of the high amount of plastics in the environment.
Paola Grenni and Anna Barra Caracciolo, Italian National Research Council’s Water Research Institute; Andrea Franzetti, Milano–Bicocca University; and Luca Alberti, Polytechnic University of Milan
The accidental spills of contaminants such as hydrocarbons and chlorinated solvents can contaminate soil and water, raising concern because they are recalcitrant compounds and toxic for ecosystems and human health. In situ remediation techniques, in particular those involving microbial communities, represent an economically and environmentally sustainable strategy for reducing the contamination and understanding the responses of biological targets to these pollutants. The development of new tools and technologies for monitoring in situ and enhancing microbial activity is crucial for advancing the remediation of contaminated sites. In order to make this technology more profitable and successful, it is important to include an interdisciplinary involvement of scientists of different discipline (e.g. hydrogeologists, microbiologists, chemists and ecotoxicologists).
This session, incorporated several research applications, embracing soil, sediments and groundwater matrices, including six oral presentations and 22 posters. The use of innovative methods for both monitoring and enhancing the bioremediation strategy were presented. Several presentations focused on the stimulation of biodegradation processes, others on advances in the exploitation of plant-bacteria interactions, moreover the use of colloidal activated carbons and bio-electrochemical approaches were also presented.
Sara Borin, University of Milan, described the biostimulation performed by different plant species in a soil polluted by polychlorinated biphenyls (PCBs). Marco Petrangeli Papini, Rome La Sapienza University, reportedon the reductive dechlorination process and its ability to restoretwo aquifers contaminated by chlorinated aliphatic hydrocarbons. Federico Aulenta, Italian National Research Council’s Water Research Institute of Rome, illustrated microbial electrochemical technologies and its application for in situ treatment of a petroleum-contaminated groundwater. Other oral presentations addressed the application of novel monitoring methods, reporting new insight into biomolecular and microscope analyses. The other three oral presentations addressed the application of novel monitoring methods, reporting new insight into biomolecular and microscope analyses. Joaquim Vila, Spanish Institute of Natural Resources and Agrobiology, described microbial processes linked to polycyclic aromatic hydrocarbons (PAH) biodegradation during the laboratory assessmentof a creosote-contaminated soil using the next generation sequencing technology on both DNA and RNA transcripts. Natalia P. Ivleva, Munich Technical University, presented on the use of Raman microspectroscopy in combination with a stable isotope approach for the characterization of the molecular and isotopic composition of microorganisms at the single cell level. Finally, Renato Baciocchi, Rome Tor Vergata University, demonstrated that the calculation of the natural attenuation rates for petroleum hydrocarbons using the flux of contaminants by assessing the effective emission rate of the contaminants measured with the flux chambers.
The poster session was extensive with 22 presentations, and topics included recent applications for restoring ecosystems affected by various contaminants such as PCBs, dichlorodiphenyldichloroethene (DDE), hexavalent chromium and various other hydrocarbons, including polycyclic aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, polyfluorinated alkyl substances and monochlorobenzenes. Several approaches for monitoring and evaluating bioremediation processes, such as the use of biomolecular tools, compound specific isotope analysis and software, were presented. Both senior researchers and students actively participated in the session.
In both the poster and platform presentations, microbes were in the spotlight, due to their great versatility in contaminant degradation and the many efforts that have been made to promote them, in particular in addressing persistent organic contaminants. The importance of this session was due to the fact that it used a multidisciplinary approach to discuss techniques in onsite applications to enhance microbial activity using in situ rather than laboratory only experiments.
In summary, while there are several organic pollutants no longer in production, such as PCBs, they still constitute an environmental problem due to their persistence in the environment. Many studies have been performed to restore sites from the effects of contamination, but additional research is still necessary, in particular site-specific interdisciplinary research to widen the range of pollutants treated by biological methods and to increase the efficiency of in situ applications.
Summaries Published in the June 2018 Issue
Ana Marta Gonçalves, University of Aveiro; Nelson Abrantes, University of Aveiro; and Alice Horton and Claus Svendsen, Centre for Ecology and Hydrology, UK
The accumulation of plastics in freshwater and terrestrial systems constitutes an emerging scientific and societal issue due to their ubiquity, high persistence and potential to cause ecological effects. As a modern, populous society, we rely heavily on the preservation of these environments as resources for both agriculture and drinking water. The factors influencing fate, behavior and effects of particles are complex and can rely on a combination of environmental and biological interactions. The form in which plastics enter the environment will have a significant influence on their behavior.
In most cases, the results of several studies are not comparable due to the application of different methods of sampling, processing and analysis. This session covered distinct aspects of extraction and methodological approaches, fate, monitoring and effects of microplastics in freshwater and terrestrial systems.
Morning Slot 1
This first slot was focused mainly on novel methodologies and analytical tools for detection and quantification of micro- and nanoplastics within the environment. The presentations detailed spectroscopic and chemical analytical tools to detect, identify the types of polymers and quantify microplastics in samples, including a new metal-doping technique for particle tracing. The presentations included extraction techniques for complex media such as soil, sludge and wastewater. The presentations provided data on microplastic distributions within terrestrial environments such as sludge-treated land. The final presentation considered how particle properties may influence their behavior within aquatic systems.
Morning Slot 2
The second slot was predominantly focused on data from environmental surveys of rivers and storm water ponds, which may both accumulate microplastics from various sources, influenced by environmental factors. The presentations also considered modeling as a tool for predicting particle interactions with other pollutants. As part of this session, we also had a poster spotlight session where three poster presenters gave a brief overview of their work.
The afternoon focused on modeling tools for predicting the transport, fate and behavior of microplastics, in addition to predicting how particle properties can affect their interactions and biological effects to organisms. Varied presentations studied bioaccumulation, biochemical and molecular effects. This session also included three poster spotlights, which were selected to represent the breadth of the research presented in the session.
Overall, this session highlighted the wide range of research that is currently being carried out in the field of microplastics, with several novel techniques being developed and implemented, and a rapidly increasing amount of data. However, land systems are still underrepresented when it comes to environmental studies due to methodological difficulties when working with complex samples. There is still a need for standardization of methods to allow for comparability between studies, which the ongoing research will address.
Jean-Pierre Desforges, Aarhus University; Elke Zimmer, ibacon GmbH; and Andre Gergs, Bayer AG
A major challenge for ecotoxicologists is to understand the complex real-world situation in which terrestrial and aquatic organisms are dynamically exposed to multiple chemical and environmental stressors. There is increasing interest from stakeholders to develop mechanistic models for risk assessment that focus on the underlying processes governing environmental or physiological changes to extrapolate across levels and make predictions for untested scenarios. Integrated approaches utilizing the vast “toolbox” in ecotoxicology are needed to better understand the links between exposure and biological effects of contaminants and other stressors. At the SETAC Europe 28th Annual Meeting in Rome, we chaired a session to broaden our understanding of how process-based modeling can be used to advance the interpretation of and predictions from laboratory and environmental toxicology studies.
The use of ecological and process-based modeling has advanced considerably in recent years, as evidenced by the broad array of contaminant types, toxicity endpoints, modeling approaches and species covered during the session talks and discussions. The oral and poster presentations centered around three main themes: toxicokinetic-toxicodynamic (TKTD) models of contaminants, full life cycle risk assessment using Dynamic Energy Budgets (DEB) and extrapolating effects across biological levels. Here we briefly summarize the major themes discussed within this session.
- DEB theory emerged as an especially useful framework to evaluate the effects of multiple stressors on organisms, considering various health and toxicity endpoints, timescales and life history traits. Sylvain Bart, INRA France, experimentally exposed earthworms to a copper fungicide and used DEB to model effects on growth and reproduction throughout ontogeny. Erik Muller, University of California, modeled bioaccumulation and sub-lethal and lethal effects of nano-particles in amphipods. A framework was also presented by Louise Stevenson, University of California–Santa Barbara, to combine molecular information (AOPs) in DEB models of organisms. Such an approach opens the door for future collaborations between ecological modelers and molecular toxicologists. Finally, several posters presented interesting DEB applications in copepods, fish and marine mammals.
- The importance of TKTD models in risk assessments was highlighted in several talks and posters. Sandrine Charles, Université de Lyon, started the session with an overview of TKTD models and their use in risk assessment and presented a new R package “morse” that simplifies user implementation of TKTD models. The application of TKTD modeling in multiple stressor situations was explored by Yen Le (University of Duisburg-Essen, Germany) in her talk about metal accumulation in fish infected with acanthocephalan parasites. Several posters focused on this issue, presenting new initiatives for the General Unified Threshold model for Survival (GUTS) and metal uptake and partitioning within cells.
- There is increasing interest in ecotoxicology to understand how contaminant effects extrapolate between biological levels and complexity. Irv Schultz, U.S. National Oceanic and Atmospheric Administration, showed how biologically based mathematical models can be used to extrapolate in vitro data for endocrine active contaminants in rainbow trout, linking molecular data to reproductive performance. We also had several posters examining quantitative AOP models, including endpoints for lung cancer, embryotoxicity and recruitment, and acetylcholinesterase.
In summary, the session addressed a diverse array of modeling approaches in the study of laboratory and environmental toxicology. It was clear from the session talks and discussions that process-based models are of interest to a broad group of research areas. We can expect much future development and implementation of mechanistic models in risk assessment of various stressors, especially linking sub-lethal effects to relevant biologically meaningful organism endpoints.
Peter Simpson, ECHA; Ian Cousins, Stockholm Unversity; Patrik Andersson, Omea University; and Hugo Waeterschoot, Eurometraux
There is growing recognition for the role of informed substitution as part of the risk management of chemicals, particularly for those substances considered to be of greatest concern, such as those that are carcinogenic, mutagenic or reprotoxic (CMR); have endocrine disrupting (ED) properties; or are persistent, bioaccumulative and toxic (PBT). This recognition is driven by international conventions, such as the Stockholm Convention, as well as regional regulatory systems (e.g., the REACH authorization and restriction scheme), national legislation, non-government organizations or, in some cases, by consumers themselves. There is also a growing interest and motivation to substitute hazardous chemicals within industry. All are seeking guidance on the selection of appropriate methods and tools for chemical substitution.
A number of case studies were presented during the oral and poster sessions, including focus on the alternatives review process for perfluorooctanesulfonate (PFOS) at international levels under the Stockholm Convention; alternatives assessment for regional regulatory risk-based assessment, such as lead in gunshot in wetlands; practical programs on the assessment of alternatives for certain per- and polyfluoroalkyl substances (PFAS) used in durable water repellent (DWR) fabrics; recycling practices of textile materials and non-ferrous metals; and substitution of flame retardants, PFAS in fire-fighting foams and chemicals in food packaging. Some overarching themes from these contributions were:
- A proper understanding of the functionality of the substance is key to assess the relevancy for substitution and the starting base.
- Alternatives should be defined, recognizing the technical performance for each of the functional uses. This may result in alternatives for some functionalities while not for others.
- Regrettable substitution should be prevented, which requires a balanced view on the toxicity or risks of the alternatives. It also requires taking a holistic perspective covering the impact of the product during its entire life cycle and recycling after “End of Life” when considering circular economy perspectives.
- Life Cycle Impact Assessment (LCIA) models and approaches could help in this respect but may not always be adapted to the type of chemicals being assessed. Therefore, their application requires careful consideration. Science will play an important role in ensuring that appropriate models are available in time.
- LCIA, hazard and risk assessment provide complementary information that should be considered in a balanced way.
- The drivers in market versus regulatory substitution may not be equal, but both require scientific evidence.
- The road to developing alternatives between investigation and commercialization may be difficult and long, but it can be facilitated if supported by the market and if the appropriate science is available.
There was support for continuing this theme at next year’s SETAC Europe meeting in Helsinki, potentially building on the themes introduced during this session, and further development in the areas of benign-by-design and green chemistry.
Monia Niero, Aalborg University, and Eric Van Genderen, International Zinc Association
In recent years, the vision of a circular economy has rapidly gained a key role in the international political and business agendas on how to decouple economic growth from resource constraints. According to the EU Action Plan for the circular economy (2015), “The transition to a more circular economy, where the value of products, materials and resources is maintained in the economy for as long as possible, and the generation of waste minimized, is an essential contribution to the EU’s efforts to develop a sustainable, low carbon, resource efficient and competitive economy.”
To this end, material producers and downstream users are being asked to provide ever greater and more sophisticated information to authorities and consumers on the environmental footprints of the materials it produces and markets. For a circular economy to be sustainable, circularity strategies (i.e., strategies aiming at keeping products, components and materials at their highest utility at all times), need to also consider economic, social and normative aspects beyond the environmental considerations.
In such an articulated context, what is the role for life cycle approaches in the definition of a sustainable circular economy? As life cycle approaches are very powerful methodologies to address trade-offs, both between life cycle stages and different sustainability aspects, the purpose of this session was to facilitate information exchange on methodological developments and applications of stand-alone or integrated life cycle approaches (environmental footprint, life cycle sustainability assessment), present challenges associated with cultural barriers for data collection, and illustrate the need for a collective response from all stakeholders. Presentations covered aspects of the transition to a sustainable circular economy, from both methodological and applicative perspectives, and provided case studies in different areas focusing on the following themes:
The Role of Indicators to Measure Circularity
Hanna Helander, University of Freiburg, presented a framework to compare and assess circular economy performance indicators at a product level based on the life cycle phase (production, use phase, end-of-life), and Monia Niero, Aalborg University, discussed a case in the packaging sector where multicriteria decision analysis is used in combination with different material circularity and life cycle based indicators.
Methodological Challenges for Life Cycle Assessment (LCA)
Dieuwertje Schrijvers, University of Bordeaux, addressed the importance of defining the proper research question and derived archetypes of LCA goal and scope definition based on several criteria (perspective, reason to conduct the study, allocation procedure).
David Turner, EMPA, emphasized the crucial role of introducing region-specific life cycle inventory for tailings disposal in order to provide a more comprehensive and adequate integration of primary metals in LCA studies.
Markus Berger, TU Berlin, and Thomas Sonderegger, ETH Zurich, presented the outcomes of the task force on primary material resources in life cycle impact assessment (LCIA) and the range of LCIA methods which are more suited according to the goal and scope of a particular study.
Circular Economy Strategies Other Than Recycling
Ellen Bracquené, KU Leuven, focused on the reuse strategy and addressed the potential benefits of repair and reuse of products and components in the case of laptops, highlighting the key role of durability. Meanwhile Martí Rufí, Universitat Autònoma de Barcelona, addressed the challenge of closing loops in the biological cycle, namely in the case of integrated rooftop greenhouses.
Raoul Meys, RWTH Aachen, discussed under which conditions chemical recycling of polyethylene terephthalate (PET) bottles is favorable from an environmental point of view, in comparison with mechanical recycling. Meanwhile Luca Ciacci, University of Bologna, presented a scenario analysis with material flow analysis of copper in Europe, highlighting the potentials for greenhouse gases emissions reduction.
Coupling of Environmental and Economic Methods
Changgun Lee, University College London, presented a multi-objective, mixed-integer, linear programming optimization problem considering different reverse supply chain network configurations to close the loop in the case of fluorescent lamps. Benoit Timmermans and Wouter Achten, Université Libre de Bruxelles, discussed how LCA could be used to adjust consumption taxes, introducing the Damage and Value Added Tax (DaVAT) concept.
The session made clear that life cycle approaches are essential to move towards a sustainable circular economy, although most of the contribution focused on the environmental and, to a limited extent, economic aspects, disregarding the social aspects.
More efforts are needed to adapt the LCA methodology as a decision support tool to assess circularity strategies in all phases (goal and scope definition, life cycle inventory, LCIA and interpretation).
Elizabeth Collison, APC, Wetherby; Jacobijn van Etten, Ctgb; and Alison Hamer, ERM Group, Inc.
With a total of 13 poster presentations by authors from academia, industry and regulatory authorities, this session highlighted the continuing interest and relevance of biopesticides to the ecotoxicology community.
The poster corner session focused on microbial biopesticides, regulated in Europe under Regulation (EC) 1107/2009. Through dialogue with the presenters and the wider audience, we aimed to identify the key challenges currently faced by applicants, study directors or evaluators during the evaluation process of microbial active substances and products. This proved to be a valuable discussion as a common theme emerged. The consensus among the 20 or so participants was that a particular ecotoxicological test type or non-target organism group was not of key concern, but rather across test types a more structured approach is needed for microbials to account for the different requirements in test design and interpretation compared with conventional chemical substances. Those participating in the poster corner session felt that this was where most of the experience currently, and understandably, lies. After carefully designing their study program, notifiers need reassurance that their approaches will be accepted by all concerned authorities, and the current guidelines are not yet sufficient for this purpose and are often open to interpretation with no clear goal around study acceptance criteria. It was noted that the end solution should not just be a set of test guidelines fit for purpose for microbials but a better understanding of how endpoints and data generated are used in the risk assessment process. Concerns raised included how to measure and interpret potential effects of infectivity and pathogenicity on non-target organisms, what application rates and test item doses can be practically tested, and how to verify exposure and dosing concentrations. Further discussions included whether we are currently assessing appropriate organism groups and whether a qualitative approach based on the characteristics of the microorganism might be more suitable than quantitative risk assessments.
We hope this first SETAC session on the topic highlighted that there are shared challenges to overcome, with an acknowledgement that all stakeholders (industry notifiers, study directors, regulators and researchers) can contribute to developing appropriate solutions. SETAC provides a valuable platform for these discussions, and we consider that this session brought an interested network together and formed a valuable starting point for a continuation of the dialogue at future meetings.
Nadezhda Kudryasheva, Institute of Biophysics, Siberian Branch of Russian Academy of Sciences; and Valentina Kratasyuk, Siberian Federal University
Analytical methods utilizing luminescence have been developed in many areas of biology, medicine and ecology. Luminescence is a highly useful tool since it can be detected with simple physics devices. This type of analysis is not time consuming, it provides a lot of experimental information under comparable conditions, which is essential for further evaluation using statistical tools.
Excited states in luminescent systems can be obtained in many different ways, such as chemical reactions (chemiluminescence), biochemical reactions (bioluminescence) and photoexcitation (photoluminescence). Bioluminescence provides an opportunity in using the assay systems in ecotoxicology. Chemiluminescene methods reveals a role of reactive oxygen species, and photoluminescence provides a study of physico-chemical processes taking place in complex systems, including living organisms.
This session presented reports from researchers working with luminous organisms (bacteria) using enzyme reactions and fluorescent proteins, chemiluminescent reactions for study peroxide content, and fluorescence of intracellular components of fungi spores and fluorescence of surface of nanoparticles. Fundamental and applied aspects were encouraged, including ecological monitoring of waste and fresh waters, soils and air; application of luminescent bioassays in remediation procedures; and the luminescent ecological biosensors including instrumentation.
The key points for the five presentations included:
- Valentina Kratasyuk, Siberian Federal University, discussed a development of bacterial bioluminescent enzymatic biosensors and their application as toxicity bioassays. New enzymatic reagents and portable devices (bioluminometers) were presented.
- Nadezhda Kudryasheva, Institute of Biophysics Russian Academy of Sciences, Siberian Federal University, presented an investigation of toxic and adaptive effects using luminescent assay systems of different complexity: bacterial cells, enzyme reactions and fluorescent proteins.
- Aleksei Trofimov, Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, presented a new application of chemiluminescent method for a comparative study on oxidant and antioxidant activity of two types of nicotine-containing aerosols, namely tobacco (cigarette) smoke and emissions derived from electronic cigarettes. It was concluded that electronic cigarettes and tobacco cigarettes exert different oxidative impacts on humans.
- Investigation of fluorescence spectra of filamentous fungi cultivated under different concentrations of source of bioavailable and not readily bioavailable carbon in the growth medium was presented by Fedoseeva Elena, Pirogov Russian National Research Medical University, Russia. Fluorescence characteristics were found to correlate with saturation of growth medium by sources of carbon. This research is promising for using fungal fluorophores to assess responses to external stimuli.
- New fluorescence method to investigate the surface characteristics of nanoparticles was presented by Lyubov Kulyabko, Moscow Aviation Institute. Relations of the surface characteristics with bioactivity of the nanoparticles Fe3O4 was demonstrated. Kulyabko hypothesized that, along with the size of nanoparticles, coordination of ligands on their surface is a major factor contributing to the toxicity of nanoparticles.
The session included three poster spotlights by Marie-Claire Lot, CEHTRA; Anna Sachkova, Tomsk Polytechnic University; and Alena Petrova, Krasnoyarsk State Agrarian University. They presented a comparison between results of LumiMARA and Microtox tests, an application of bioluminescent assays for studying antioxidant activity and toxicity of bioactive compounds (humic substances and carbon nanoparticles), and the effect of low-dose gamma-radiation on luminous marine bacteria.
The ability to emit light by several organisms is widely used by researchers in toxicological investigations. Luminescence is assumed here as a physiological function, and it is sensitive to the presence of exogenous compounds. Luminescence can be used to evaluate quantitatively a toxicity of media or monitor adaptive response of organisms.
Luminescent assay systems can be based on living systems of different complexity, ranging from higher organisms to cells and specific proteins. Within the last decades, there has been substantial development of new bioassays that are based on enzymatic reactions (enzymatic bioassays). Enzymatic bioassays can be used to estimate rates of biochemical reactions under toxicant influence and provide a better understanding of the toxicity mechanisms at both the molecular and macromolecular levels. These new type of bioassays are based on fluorescent coelenteramide-containing proteins, which can reveal the effects of toxic compounds on protein structures. These bioassays lead to a more complete understanding of the physicochemical processes in biological responses to toxicants. Chemiluminescence methods have proven to be highly prospective for the evaluation of the role which reactive oxygen species have in responses to toxic effects and the resulting protective responses of organisms. Photoluminescence methods have also proven to be very useful for monitoring of intracellular processes and the properties of toxic compounds.